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-Structure paper
| タイトル | A promiscuous mechanism to phase separate eukaryotic carbon fixation in the green lineage. |
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| ジャーナル・号・ページ | Nat Plants, Vol. 10, Issue 11, Page 1801-1813, Year 2024 |
| 掲載日 | 2024年10月9日 |
著者 | James Barrett / Mihris I S Naduthodi / Yuwei Mao / Clément Dégut / Sabina Musiał / Aidan Salter / Mark C Leake / Michael J Plevin / Alistair J McCormick / James N Blaza / Luke C M Mackinder / ![]() |
| PubMed 要旨 | CO fixation is commonly limited by inefficiency of the CO-fixing enzyme Rubisco. Eukaryotic algae concentrate and fix CO in phase-separated condensates called pyrenoids, which complete up to one- ...CO fixation is commonly limited by inefficiency of the CO-fixing enzyme Rubisco. Eukaryotic algae concentrate and fix CO in phase-separated condensates called pyrenoids, which complete up to one-third of global CO fixation. Condensation of Rubisco in pyrenoids is dependent on interaction with disordered linker proteins that show little conservation between species. We developed a sequence-independent bioinformatic pipeline to identify linker proteins in green algae. We report the linker from Chlorella and demonstrate that it binds a conserved site on the Rubisco large subunit. We show that the Chlorella linker phase separates Chlamydomonas Rubisco and that despite their separation by ~800 million years of evolution, the Chlorella linker can support the formation of a functional pyrenoid in Chlamydomonas. This cross-species reactivity extends to plants, with the Chlorella linker able to drive condensation of some native plant Rubiscos in vitro and in planta. Our results represent an exciting frontier for pyrenoid engineering in plants, which is modelled to increase crop yields. |
リンク | Nat Plants / PubMed:39384944 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 2.39 - 2.77 Å |
| 構造データ | EMDB-18049, PDB-8q04: EMDB-18050, PDB-8q05: |
| 化合物 | ![]() ChemComp-HOH: |
| 由来 |
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キーワード | PLANT PROTEIN / Rubisco |
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chlorella sorokiniana (植物)
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