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TitleStructural basis for DNA recognition by a viral genome-packaging machine.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 121, Issue 33, Page e2406138121, Year 2024
Publish dateAug 13, 2024
AuthorsMaria Chechik / Sandra J Greive / Alfred A Antson / Huw T Jenkins /
PubMed AbstractDNA recognition is critical for assembly of double-stranded DNA viruses, particularly for the initiation of packaging the viral genome into the capsid. The key component that recognizes viral DNA is ...DNA recognition is critical for assembly of double-stranded DNA viruses, particularly for the initiation of packaging the viral genome into the capsid. The key component that recognizes viral DNA is the small terminase protein. Despite prior studies, the molecular mechanism for DNA recognition remained elusive. Here, we address this question by identifying the minimal site in the bacteriophage HK97 genome specifically recognized by the small terminase and determining the structure of this complex by cryoEM. The circular small terminase employs an entirely unexpected mechanism in which DNA transits through the central tunnel, and sequence-specific recognition takes place as it emerges. This recognition stems from a substructure formed by the N- and C-terminal segments of two adjacent protomers which are unstructured when DNA is absent. Such interaction ensures continuous engagement of the small terminase with DNA, enabling it to slide along the DNA while simultaneously monitoring its sequence. This mechanism allows locating and instigating packaging initiation and termination precisely at the specific sequence.
External linksProc Natl Acad Sci U S A / PubMed:39116131 / PubMed Central
MethodsEM (single particle)
Resolution2.92 - 3.0 Å
Structure data

17794

8pop

EMDB-17794: HK97 small terminase in complex with DNA after focused classification
PDB-8pop: HK97 small terminase in complex with DNA
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-17818: Complex of HK97 small terminase with DNA
Method: EM (single particle) / Resolution: 2.92 Å

Source
  • Hendrixvirus
  • escherichia phage hk97 (virus)
  • byrnievirus hk97
KeywordsDNA BINDING PROTEIN / small terminase / HK97 / bacteriophage / complex with DNA

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