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TitleEvolution of a large periplasmic disk in Campylobacterota flagella enables both efficient motility and autoagglutination.
Journal, issue, pagesDev Cell, Vol. 59, Issue 24, Page 3306-33321.e5, Year 2024
Publish dateDec 16, 2024
AuthorsEli J Cohen / Tina Drobnič / Deborah A Ribardo / Aoba Yoshioka / Trishant Umrekar / Xuefei Guo / Jose-Jesus Fernandez / Emma E Brock / Laurence Wilson / Daisuke Nakane / David R Hendrixson / Morgan Beeby /
PubMed AbstractThe flagellar motors of Campylobacter jejuni (C. jejuni) and related Campylobacterota (previously epsilonproteobacteria) feature 100-nm-wide periplasmic "basal disks" that have been implicated in ...The flagellar motors of Campylobacter jejuni (C. jejuni) and related Campylobacterota (previously epsilonproteobacteria) feature 100-nm-wide periplasmic "basal disks" that have been implicated in scaffolding a wider ring of additional motor proteins to increase torque, but the size of these disks is excessive for a role solely in scaffolding motor proteins. Here, we show that the basal disk is a flange that braces the flagellar motor during disentanglement of its flagellar filament from interactions with the cell body and other filaments. We show that motor output is unaffected when we shrink or displace the basal disk, and suppressor mutations of debilitated motors occur in flagellar-filament or cell-surface glycosylation pathways, thus sidestepping the need for a flange to overcome the interactions between two flagellar filaments and between flagellar filaments and the cell body. Our results identify unanticipated co-dependencies in the evolution of flagellar motor structure and cell-surface properties in the Campylobacterota.
External linksDev Cell / PubMed:39362219 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution25.5 - 45.2 Å
Structure data

EMDB-17769: Subtomogram average of the Campylobacter jejuni motor with basal disk genes, flgPQ, expressed at very low level
Method: EM (subtomogram averaging) / Resolution: 40.0 Å

EMDB-17770: Subtomogram average of the Campylobacter jejuni flagellar motor with the basal disk genes, flgPQ, expressed at low level
Method: EM (subtomogram averaging) / Resolution: 25.5 Å

EMDB-17771: Subtomogram average of the Campylobacter jejuni flagellar motor with the basal disk genes, flgPQ, expressed at medium level
Method: EM (subtomogram averaging) / Resolution: 39.6 Å

EMDB-17772: Subtomogram average of the Campylobacter jejuni motor with the basal disk genes, flgPQ, expressed at high level
Method: EM (subtomogram averaging) / Resolution: 43.9 Å

EMDB-17773: Subtomogram average of the Campylobacter jejuni flgP S69A E157A K159A (flgP-AAA) flagellar motor
Method: EM (subtomogram averaging) / Resolution: 34.5 Å

EMDB-17774: Subtomogram average of the Campylobacter jejuni flgP Del18-62 flagellar motor
Method: EM (subtomogram averaging) / Resolution: 39.8 Å

EMDB-17775: Subtomogram average of the Campylobacter jejuni flgPQ deletion flagellar motor
Method: EM (subtomogram averaging) / Resolution: 36.2 Å

EMDB-17776: Subtomogram average of the Campylobacter jejuni DflgPQ D0661 DkpsD DpglAB flagellar motor
Method: EM (subtomogram averaging) / Resolution: 33.2 Å

EMDB-18274: Subtomogram average of the Campylobacter jejuni FlgP-Lpp55 flagellar motor
Method: EM (subtomogram averaging) / Resolution: 45.2 Å

Source
  • Campylobacter jejuni (Campylobacter)

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