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Title | The palisade layer of the poxvirus core is composed of flexible A10 trimers. |
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Journal, issue, pages | Nat Struct Mol Biol, Year 2024 |
Publish date | Feb 5, 2024 |
Authors | Jiasui Liu / Simon Corroyer-Dulmont / Vojtěch Pražák / Iskander Khusainov / Karola Bahrami / Sonja Welsch / Daven Vasishtan / Agnieszka Obarska-Kosińska / Sigurdur R Thorkelsson / Kay Grünewald / Emmanuelle R J Quemin / Beata Turoňová / Jacomina Krijnse Locker / |
PubMed Abstract | Due to its asymmetric shape, size and compactness, the structure of the infectious mature virus (MV) of vaccinia virus (VACV), the best-studied poxvirus, remains poorly understood. Instead, subviral ...Due to its asymmetric shape, size and compactness, the structure of the infectious mature virus (MV) of vaccinia virus (VACV), the best-studied poxvirus, remains poorly understood. Instead, subviral particles, in particular membrane-free viral cores, have been studied with cryo-electron microscopy. Here, we compared viral cores obtained by detergent stripping of MVs with cores in the cellular cytoplasm, early in infection. We focused on the prominent palisade layer on the core surface, combining cryo-electron tomography, subtomogram averaging and AlphaFold2 structure prediction. We showed that the palisade is composed of densely packed trimers of the major core protein A10. Trimers display a random order and their classification indicates structural flexibility. A10 on cytoplasmic cores is organized in a similar manner, indicating that the structures obtained in vitro are physiologically relevant. We discuss our results in the context of the VACV replicative cycle, and the assembly and disassembly of the infectious MV. |
External links | Nat Struct Mol Biol / PubMed:38316878 |
Methods | EM (subtomogram averaging) |
Resolution | 7.7 - 13.4 Å |
Structure data | EMDB-17704: Subtomogram average of Vaccinia A10 trimer with open center from in vitro cores EMDB-17708: Subtomogram average of Vaccinia A10 trimer with tight center from in vitro cores EMDB-17753: Subtomogram average of Vaccinia A10 trimer from in situ cores |