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TitleRNA channelling by the eukaryotic exosome.
Journal, issue, pagesEMBO Rep, Vol. 11, Issue 12, Page 936-942, Year 2010
Publish dateNov 12, 2010
AuthorsHélène Malet / Maya Topf / Daniel K Clare / Judith Ebert / Fabien Bonneau / Jerome Basquin / Karolina Drazkowska / Rafal Tomecki / Andrzej Dziembowski / Elena Conti / Helen R Saibil / Esben Lorentzen /
PubMed AbstractThe eukaryotic exosome is a key nuclease for the degradation, processing and quality control of a wide variety of RNAs. Here, we report electron microscopic reconstructions and pseudo-atomic models ...The eukaryotic exosome is a key nuclease for the degradation, processing and quality control of a wide variety of RNAs. Here, we report electron microscopic reconstructions and pseudo-atomic models of the ten-subunit Saccharomyces cerevisiae exosome in the unbound and RNA-bound states. In the RNA-bound structures, extra density that is visible at the entry and exit sites of the exosome channel indicates that a substrate-threading mechanism is used by the eukaryotic exosome. This channelling mechanism seems to be conserved in exosome-like complexes from all domains of life, and might have been present in the most recent common ancestor.
External linksEMBO Rep / PubMed:21072061 / PubMed Central
MethodsEM (single particle)
Resolution12.0 - 14.0 Å
Structure data

EMDB-1708:
Cryo-EM structure of S. cerevisiae 10-subunit exosome
Method: EM (single particle) / Resolution: 14.0 Å

EMDB-1709:
Cryo-EM structure of S. cerevisiae 10-subunit exosome bound to RNA
Method: EM (single particle) / Resolution: 12.0 Å

Source
  • Saccharomyces cerevisiae (brewer's yeast)
  • synthetic construct (others)

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