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-Structure paper
タイトル | Determining the effects of pseudouridine incorporation on human tRNAs. |
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ジャーナル・号・ページ | EMBO J, Vol. 44, Issue 13, Page 3553-3585, Year 2025 |
掲載日 | 2025年4月29日 |
![]() | Anna D Biela / Jakub S Nowak / Artur P Biela / Sunandan Mukherjee / Seyed Naeim Moafinejad / Satyabrata Maiti / Andrzej Chramiec-Głąbik / Rahul Mehta / Jakub Jeżowski / Dominika Dobosz / Priyanka Dahate / Veronique Arluison / Frank Wien / Paulina Indyka / Michal Rawski / Janusz M Bujnicki / Ting-Yu Lin / Sebastian Glatt / ![]() ![]() ![]() ![]() |
PubMed 要旨 | Transfer RNAs (tRNAs) are ubiquitous non-coding RNA molecules required to translate mRNA-encoded sequence information into nascent polypeptide chains. Their relatively small size and heterogenous ...Transfer RNAs (tRNAs) are ubiquitous non-coding RNA molecules required to translate mRNA-encoded sequence information into nascent polypeptide chains. Their relatively small size and heterogenous patterns of their RNA modifications have impeded the systematic structural characterization of individual tRNAs. Here, we use single-particle cryo-EM to determine the structures of four human tRNAs before and after incorporation of pseudouridines (Ψ). Following post-transcriptional modifications by distinct combinations of human pseudouridine synthases, we find that tRNAs become stabilized and undergo specific local structural changes. We establish interactions between the D- and T-arms as the key linchpin in the tertiary structure of tRNAs. Our structures of human tRNAs highlight the vast potential of cryo-EM combined with biophysical measurements and computational simulations for structure-function analyses of tRNAs and other small, folded RNA domains. |
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手法 | EM (単粒子) |
解像度 | 5.26 Å |
構造データ | ![]() EMDB-16950: cryo-EM structure of human tRNA(AspGUC) |
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