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TitleAn expandable, modular de novo protein platform for precision redox engineering.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 120, Issue 31, Page e2306046120, Year 2023
Publish dateJul 24, 2023
AuthorsGeorge H Hutchins / Claire E M Noble / H Adrian Bunzel / Christopher Williams / Paulina Dubiel / Sathish K N Yadav / Paul M Molinaro / Rob Barringer / Hector Blackburn / Benjamin J Hardy / Alice E Parnell / Charles Landau / Paul R Race / Thomas A A Oliver / Ronald L Koder / Matthew P Crump / Christiane Schaffitzel / A Sofia F Oliveira / Adrian J Mulholland / J L Ross Anderson /
PubMed AbstractThe electron-conducting circuitry of life represents an as-yet untapped resource of exquisite, nanoscale biomolecular engineering. Here, we report the characterization and structure of a de novo ...The electron-conducting circuitry of life represents an as-yet untapped resource of exquisite, nanoscale biomolecular engineering. Here, we report the characterization and structure of a de novo diheme "maquette" protein, 4D2, which we subsequently use to create an expanded, modular platform for heme protein design. A well-folded monoheme variant was created by computational redesign, which was then utilized for the experimental validation of continuum electrostatic redox potential calculations. This demonstrates how fundamental biophysical properties can be predicted and fine-tuned. 4D2 was then extended into a tetraheme helical bundle, representing a 7 nm molecular wire. Despite a molecular weight of only 24 kDa, electron cryomicroscopy illustrated a remarkable level of detail, indicating the positioning of the secondary structure and the heme cofactors. This robust, expressible, highly thermostable and readily designable modular platform presents a valuable resource for redox protein design and the future construction of artificial electron-conducting circuitry.
External linksProc Natl Acad Sci U S A / PubMed:37487099 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution1.91 - 8.4 Å
Structure data

EMDB-16847: CryoEM structure of holo e4D2
Method: EM (single particle) / Resolution: 8.4 Å

PDB-7ah0:
Crystal structure of the de novo designed two-heme binding protein, 4D2
Method: X-RAY DIFFRACTION / Resolution: 1.91 Å

PDB-8ccr:
Crystal structure of the T19D mutant of the de novo diheme binding 4D2
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

Chemicals

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE / Heme B

ChemComp-HOH:
WATER / Water

ChemComp-LMR:
(2S)-2-hydroxybutanedioic acid / Malic acid

ChemComp-CL:
Unknown entry / Chloride

Source
  • synthetic construct (others)
  • escherichia coli (E. coli)
KeywordsDE NOVO PROTEIN / Heme / Maquette / ELECTRON TRANSPORT / Heme binding / De Novo

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