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TitleInterdigitated immunoglobulin arrays form the hyperstable surface layer of the extremophilic bacterium .
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 120, Issue 16, Page e2215808120, Year 2023
Publish dateApr 18, 2023
AuthorsAndriko von Kügelgen / Sofie van Dorst / Keitaro Yamashita / Danielle L Sexton / Elitza I Tocheva / Garib Murshudov / Vikram Alva / Tanmay A M Bharat /
PubMed Abstract is an atypical diderm bacterium with a remarkable ability to tolerate various environmental stresses, due in part to its complex cell envelope encapsulated within a hyperstable surface layer (S- ... is an atypical diderm bacterium with a remarkable ability to tolerate various environmental stresses, due in part to its complex cell envelope encapsulated within a hyperstable surface layer (S-layer). Despite decades of research on this cell envelope, atomic structural details of the S-layer have remained obscure. In this study, we report the electron cryomicroscopy structure of the S-layer, showing how it is formed by the Hexagonally Packed Intermediate-layer (HPI) protein arranged in a planar hexagonal lattice. The HPI protein forms an array of immunoglobulin-like folds within the S-layer, with each monomer extending into the adjacent hexamer, resulting in a highly interconnected, stable, sheet-like arrangement. Using electron cryotomography and subtomogram averaging from focused ion beam-milled cells, we have obtained a structure of the cellular S-layer, showing how this HPI S-layer coats native membranes on the surface of cells. Our S-layer structure from the diderm bacterium shows similarities to immunoglobulin-like domain-containing S-layers from monoderm bacteria and archaea, highlighting common features in cell surface organization across different domains of life, with connotations on the evolution of immunoglobulin-based molecular recognition systems in eukaryotes.
External linksProc Natl Acad Sci U S A / PubMed:37043530 / PubMed Central
MethodsEM (single particle)
Resolution2.52 Å
Structure data

16694

8cka

EMDB-16694, PDB-8cka:
Deinococcus radidurans HPI S-layer
Method: EM (single particle) / Resolution: 2.52 Å

Source
  • Deinococcus radiodurans (radioresistant)
  • deinococcus radiodurans (strain atcc 13939 / dsm 20539 / jcm 16871 / lmg 4051 / nbrc 15346 / ncimb 9279 / r1 / vkm b-1422) (radioresistant)
KeywordsSTRUCTURAL PROTEIN / HPI S-layer

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