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-Structure paper
タイトル | Phage proteins target and co-opt host ribosomes immediately upon infection. |
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ジャーナル・号・ページ | Nat Microbiol, Vol. 9, Issue 3, Page 787-800, Year 2024 |
掲載日 | 2024年3月4日 |
著者 | Milan Gerovac / Kotaro Chihara / Laura Wicke / Bettina Böttcher / Rob Lavigne / Jörg Vogel / |
PubMed 要旨 | Bacteriophages must seize control of the host gene expression machinery to replicate. To bypass bacterial anti-phage defence systems, this host takeover occurs immediately upon infection. A general ...Bacteriophages must seize control of the host gene expression machinery to replicate. To bypass bacterial anti-phage defence systems, this host takeover occurs immediately upon infection. A general understanding of phage mechanisms for immediate targeting of host transcription and translation processes is lacking. Here we introduce an integrative high-throughput approach to uncover phage-encoded proteins that target the gene expression machinery of Pseudomonas aeruginosa immediately upon infection with the jumbo phage ΦKZ. By integrating biochemical, genetic and structural analyses, we identify an abundant and conserved phage factor ΦKZ014 that targets the large ribosomal subunit by binding the 5S ribosomal RNA, and rapidly promotes replication in several clinical isolates. ΦKZ014 is among the earliest ΦKZ proteins expressed after infection and remains bound to ribosomes during the entire translation cycle. Our study provides a strategy to decipher molecular components of phage-mediated host takeover and argues that phage genomes represent an untapped discovery space for proteins that modulate the host gene expression machinery. |
リンク | Nat Microbiol / PubMed:38443577 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.0 Å |
構造データ | EMDB-16566: 70S-PHIKZ014 PHIKZ phage protein occupied ribosome |
化合物 | ChemComp-MG: |
由来 |
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キーワード | RIBOSOME (リボソーム) / PHIKZ / Phage (ファージ) / hijacked / bacterial (細菌) / Pseudomonas (シュードモナス属) / PHIKZ014 / 5S rRNA (5SリボソームRNA) |