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TitleCryo-EM structure and function of S. pombe complex IV with bound respiratory supercomplex factor.
Journal, issue, pagesCommun Chem, Vol. 6, Issue 1, Page 32, Year 2023
Publish dateFeb 16, 2023
AuthorsAgnes Moe / Pia Ädelroth / Peter Brzezinski / Linda Näsvik Öjemyr /
PubMed AbstractFission yeast Schizosaccharomyces pombe serves as model organism for studying higher eukaryotes. We combined the use of cryo-EM and spectroscopy to investigate the structure and function of affinity ...Fission yeast Schizosaccharomyces pombe serves as model organism for studying higher eukaryotes. We combined the use of cryo-EM and spectroscopy to investigate the structure and function of affinity purified respiratory complex IV (CIV) from S. pombe. The reaction sequence of the reduced enzyme with O proceeds over a time scale of µs-ms, similar to that of the mammalian CIV. The cryo-EM structure of CIV revealed eleven subunits as well as a bound hypoxia-induced gene 1 (Hig1) domain of respiratory supercomplex factor 2 (Rcf2). These results suggest that binding of Rcf2 does not require the presence of a CIII-CIV supercomplex, i.e. Rcf2 is a component of CIV. An AlphaFold-Multimer model suggests that the Hig1 domains of both Rcf1 and Rcf2 bind at the same site of CIV suggesting that their binding is mutually exclusive. Furthermore, the differential functional effect of Rcf1 or Rcf2 is presumably caused by interactions of CIV with their different non-Hig1 domain parts.
External linksCommun Chem / PubMed:36797353 / PubMed Central
MethodsEM (single particle)
Resolution3.36 Å
Structure data

16491

8c8q

EMDB-16491, PDB-8c8q:
Cytochrome c oxidase from Schizosaccharomyces pombe
Method: EM (single particle) / Resolution: 3.36 Å

Chemicals

ChemComp-HEA:
HEME-A / Heme A

ChemComp-CU:
COPPER (II) ION / Copper

ChemComp-MG:
Unknown entry

ChemComp-CA:
Unknown entry

ChemComp-PEF:
DI-PALMITOYL-3-SN-PHOSPHATIDYLETHANOLAMINE / phospholipid*YM / Phosphatidylethanolamine

ChemComp-CUA:
DINUCLEAR COPPER ION

ChemComp-ZN:
Unknown entry

Source
  • schizosaccharomyces pombe (fission yeast)
  • fission yeast (fission yeast)
KeywordsMEMBRANE PROTEIN / Electron transfer / proton transfer / respiration

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