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TitleImmunoglobulin germline gene polymorphisms influence the function of SARS-CoV-2 neutralizing antibodies.
Journal, issue, pagesImmunity, Vol. 56, Issue 1, Page 193-206.e7, Year 2023
Publish dateJan 10, 2023
AuthorsPradeepa Pushparaj / Andrea Nicoletto / Daniel J Sheward / Hrishikesh Das / Xaquin Castro Dopico / Laura Perez Vidakovics / Leo Hanke / Mark Chernyshev / Sanjana Narang / Sungyong Kim / Julian Fischbach / Simon Ekström / Gerald McInerney / B Martin Hällberg / Ben Murrell / Martin Corcoran / Gunilla B Karlsson Hedestam /
PubMed AbstractThe human immunoglobulin heavy-chain (IGH) locus is exceptionally polymorphic, with high levels of allelic and structural variation. Thus, germline IGH genotypes are personal, which may influence ...The human immunoglobulin heavy-chain (IGH) locus is exceptionally polymorphic, with high levels of allelic and structural variation. Thus, germline IGH genotypes are personal, which may influence responses to infection and vaccination. For an improved understanding of inter-individual differences in antibody responses, we isolated SARS-CoV-2 spike-specific monoclonal antibodies from convalescent health care workers, focusing on the IGHV1-69 gene, which has the highest level of allelic variation of all IGHV genes. The IGHV1-6920-using CAB-I47 antibody and two similar antibodies isolated from an independent donor were critically dependent on allele usage. Neutralization was retained when reverting the V region to the germline IGHV1-6920 allele but lost when reverting to other IGHV1-69 alleles. Structural data confirmed that two germline-encoded polymorphisms, R50 and F55, in the IGHV1-69 gene were required for high-affinity receptor-binding domain interaction. These results demonstrate that polymorphisms in IGH genes can influence the function of SARS-CoV-2 neutralizing antibodies.
External linksImmunity / PubMed:36574772 / PubMed Central
MethodsEM (single particle)
Resolution2.4 - 2.7 Å
Structure data

15269

8a94

EMDB-15269: SARS CoV2 Spike in the 2-up state in complex with Fab47
PDB-8a94: SARS CoV2 Spike in the 2-up state in complex with Fab47.
Method: EM (single particle) / Resolution: 2.4 Å

15270

8a95

EMDB-15270, PDB-8a95:
SARS Cov2 Spike RBD in complex with Fab47
Method: EM (single particle) / Resolution: 2.64 Å

15271

8a96

EMDB-15271, PDB-8a96:
SARS Cov2 Spike RBD in complex with Fab47
Method: EM (single particle) / Resolution: 2.7 Å

15273

8a99

EMDB-15273, PDB-8a99:
SARS Cov2 Spike in 1-up conformation complex with Fab47
Method: EM (single particle) / Resolution: 2.5 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

Source
  • homo sapiens (human)
  • severe acute respiratory syndrome coronavirus
  • severe acute respiratory syndrome coronavirus 2
KeywordsVIRAL PROTEIN / Spike / Antibody / SARS-CoV2

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