Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	In situ architecture of the ciliary base reveals the stepwise assembly of intraflagellar transport trains.
Journal, issue, pages	Science, Vol. 377, Issue 6605, Page 543-548, Year 2022
Publish date	Jul 29, 2022
Authors	Hugo van den Hoek / Nikolai Klena / Mareike A Jordan / Gonzalo Alvarez Viar / Ricardo D Righetto / Miroslava Schaffer / Philipp S Erdmann / William Wan / Stefan Geimer / Jürgen M Plitzko / Wolfgang Baumeister / Gaia Pigino / Virginie Hamel / Paul Guichard / Benjamin D Engel /
PubMed Abstract	The cilium is an antenna-like organelle that performs numerous cellular functions, including motility, sensing, and signaling. The base of the cilium contains a selective barrier that regulates the ...The cilium is an antenna-like organelle that performs numerous cellular functions, including motility, sensing, and signaling. The base of the cilium contains a selective barrier that regulates the entry of large intraflagellar transport (IFT) trains, which carry cargo proteins required for ciliary assembly and maintenance. However, the native architecture of the ciliary base and the process of IFT train assembly remain unresolved. In this work, we used in situ cryo-electron tomography to reveal native structures of the transition zone region and assembling IFT trains at the ciliary base in . We combined this direct cellular visualization with ultrastructure expansion microscopy to describe the front-to-back stepwise assembly of IFT trains: IFT-B forms the backbone, onto which bind IFT-A, dynein-1b, and finally kinesin-2 before entry into the cilium.
External links	Science / PubMed:35901159
Methods	EM (subtomogram averaging) / EM (tomography)
Resolution	24.4 - 34.7 Å
Structure data	EMDB-15252: In situ subtomogram average of the C. reinhardtii stellate at the ciliary transition zone Method: EM (subtomogram averaging) / Resolution: 28.2 Å EMDB-15253: In situ subtomogram average of the C. reinhardtii Y-link at the ciliary transition zone Method: EM (subtomogram averaging) / Resolution: 27.9 Å EMDB-15254: In situ subtomogram average of the C. reinhardtii MTD sleeve at the ciliary transition zone Method: EM (subtomogram averaging) / Resolution: 34.7 Å EMDB-15255: In situ subtomogram average of the C. reinhardtii MTD at the ciliary transition zone Method: EM (subtomogram averaging) / Resolution: 34.0 Å EMDB-15256: Composite map of the C. reinhardtii ciliary transition zone (structures attached to a single MTD) from in situ subtomogram averaging Method: EM (subtomogram averaging) / Resolution: 28.2 Å EMDB-15257: Composite map of the C. reinhardtii ciliary transition zone (full 9-fold assembly) from in situ subtomogram averaging Method: EM (subtomogram averaging) / Resolution: 34.7 Å EMDB-15258: In situ subtomogram average of C. reinhardtii IFT-B at the ciliary transition zone Method: EM (subtomogram averaging) / Resolution: 24.4 Å EMDB-15259: In situ subtomogram average of C. reinhardtii IFT-A at the ciliary transition zone Method: EM (subtomogram averaging) / Resolution: 27.3 Å EMDB-15260: In situ subtomogram average of C. reinhardtii dynein-1b at the ciliary transition zone Method: EM (subtomogram averaging) / Resolution: 29.9 Å EMDB-15261: Composite map of the C. reinhardtii IFT (segment of a fully assembled train) at the ciliary transition zone Method: EM (subtomogram averaging) / Resolution: 29.9 Å EMDB-15262: Tomogram #3 of the C. reinhardtii ciliary transition zone Method: EM (tomography) EMDB-15263: Tomogram #12 of the C. reinhardtii ciliary transition zone Method: EM (tomography)
Source	Chlamydomonas reinhardtii (plant)