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TitleEmergence of the primordial pre-60S from the 90S pre-ribosome.
Journal, issue, pagesCell Rep, Vol. 39, Issue 1, Page 110640, Year 2022
Publish dateApr 5, 2022
AuthorsSherif Ismail / Dirk Flemming / Matthias Thoms / José Vicente Gomes-Filho / Lennart Randau / Roland Beckmann / Ed Hurt /
PubMed AbstractSynthesis of ribosomes begins in the nucleolus with formation of the 90S pre-ribosome, during which the pre-40S and pre-60S pathways diverge by pre-rRNA cleavage. However, it remains unclear how, ...Synthesis of ribosomes begins in the nucleolus with formation of the 90S pre-ribosome, during which the pre-40S and pre-60S pathways diverge by pre-rRNA cleavage. However, it remains unclear how, after this uncoupling, the earliest pre-60S subunit continues to develop. Here, we reveal a large-subunit intermediate at the beginning of its construction when still linked to the 90S, the precursor to the 40S subunit. This primordial pre-60S is characterized by the SPOUT domain methyltransferase Upa1-Upa2, large α-solenoid scaffolds, Mak5, one of several RNA helicases, and two small nucleolar RNA (snoRNAs), C/D box snR190 and H/ACA box snR37. The emerging pre-60S does not efficiently disconnect from the 90S pre-ribosome in a dominant mak5 helicase mutant, allowing a 70-nm 90S-pre-60S bipartite particle to be visualized by electron microscopy. Our study provides insight into the assembly pathway when the still-connected nascent 40S and 60S subunits are beginning to separate.
External linksCell Rep / PubMed:35385737 / PubMed Central
MethodsEM (single particle)
Resolution7.25 - 10.14 Å
Structure data

EMDB-14507: pre-60S particle (Urb2-Noc1 mak5 D333A)
Method: EM (single particle) / Resolution: 10.14 Å

EMDB-14508: 90S particle/90S-pre-60S bipartite particle (Urb2-Noc1 mak5 D333A)
Method: EM (single particle) / Resolution: 7.25 Å

Source
  • Saccharomyces cerevisiae (brewer's yeast)

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