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TitleNanobodies Protecting From Lethal SARS-CoV-2 Infection Target Receptor Binding Epitopes Preserved in Virus Variants Other Than Omicron.
Journal, issue, pagesFront Immunol, Vol. 13, Page 863831, Year 2022
Publish dateApr 25, 2022
AuthorsJosé M Casasnovas / Yago Margolles / María A Noriega / María Guzmán / Rocío Arranz / Roberto Melero / Mercedes Casanova / Juan Alberto Corbera / Nereida Jiménez-de-Oya / Pablo Gastaminza / Urtzi Garaigorta / Juan Carlos Saiz / Miguel Ángel Martín-Acebes / Luis Ángel Fernández /
PubMed AbstractThe emergence of SARS-CoV-2 variants that escape from immune neutralization are challenging vaccines and antibodies developed to stop the COVID-19 pandemic. Thus, it is important to establish ...The emergence of SARS-CoV-2 variants that escape from immune neutralization are challenging vaccines and antibodies developed to stop the COVID-19 pandemic. Thus, it is important to establish therapeutics directed toward multiple or specific SARS-CoV-2 variants. The envelope spike (S) glycoprotein of SARS-CoV-2 is the key target of neutralizing antibodies (Abs). We selected a panel of nine nanobodies (Nbs) from dromedary camels immunized with the receptor-binding domain (RBD) of the S, and engineered Nb fusions as humanized heavy chain Abs (hcAbs). Nbs and derived hcAbs bound with subnanomolar or picomolar affinities to the S and its RBD, and S-binding cross-competition clustered them in two different groups. Most of the hcAbs hindered RBD binding to its human ACE2 (hACE2) receptor, blocked cell entry of viruses pseudotyped with the S protein and neutralized SARS-CoV-2 infection in cell cultures. Four potent neutralizing hcAbs prevented the progression to lethal SARS-CoV-2 infection in hACE2-transgenic mice, demonstrating their therapeutic potential. Cryo-electron microscopy identified Nb binding epitopes in and out the receptor binding motif (RBM), and showed different ways to prevent virus binding to its cell entry receptor. The Nb binding modes were consistent with its recognition of SARS-CoV-2 RBD variants; mono and bispecific hcAbs efficiently bound all variants of concern except omicron, which emphasized the immune escape capacity of this latest variant.
External linksFront Immunol / PubMed:35547740 / PubMed Central
MethodsEM (single particle)
Resolution3.2 - 3.9 Å
Structure data

14313

7r4i

EMDB-14313, PDB-7r4i:
The SARS-CoV-2 spike in complex with the 2.15 neutralizing nanobody
Method: EM (single particle) / Resolution: 3.2 Å

14314

7r4q

EMDB-14314, PDB-7r4q:
The SARS-CoV-2 spike in complex with the 1.29 neutralizing nanobody
Method: EM (single particle) / Resolution: 3.6 Å

14315

7r4r

EMDB-14315, PDB-7r4r:
The SARS-CoV-2 spike in complex with the 1.10 neutralizing nanobody
Method: EM (single particle) / Resolution: 3.9 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

Source
  • severe acute respiratory syndrome coronavirus 2
  • camelus dromedarius (Arabian camel)
KeywordsVIRAL PROTEIN / coronavirus / COVID-19 / SARS-CoV-2 / spike / nanobodies / neutralization

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