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TitleThe Cryo-EM structures of two amphibian antimicrobial cross-β amyloid fibrils.
Journal, issue, pagesNat Commun, Vol. 13, Issue 1, Page 4356, Year 2022
Publish dateJul 27, 2022
AuthorsRobert Bücker / Carolin Seuring / Cornelia Cazey / Katharina Veith / Maria García-Alai / Kay Grünewald / Meytal Landau /
PubMed AbstractThe amyloid-antimicrobial link hypothesis is based on antimicrobial properties found in human amyloids involved in neurodegenerative and systemic diseases, along with amyloidal structural properties ...The amyloid-antimicrobial link hypothesis is based on antimicrobial properties found in human amyloids involved in neurodegenerative and systemic diseases, along with amyloidal structural properties found in antimicrobial peptides (AMPs). Supporting this hypothesis, we here determined the fibril structure of two AMPs from amphibians, uperin 3.5 and aurein 3.3, by cryogenic electron microscopy (cryo-EM), revealing amyloid cross-β fibrils of mated β-sheets at atomic resolution. Uperin 3.5 formed a 3-blade symmetrical propeller of nine peptides per fibril layer including tight β-sheet interfaces. This cross-β cryo-EM structure complements the cross-α fibril conformation previously determined by crystallography, substantiating a secondary structure switch mechanism of uperin 3.5. The aurein 3.3 arrangement consisted of six peptides per fibril layer, all showing kinked β-sheets allowing a rounded compactness of the fibril. The kinked β-sheets are similar to LARKS (Low-complexity, Amyloid-like, Reversible, Kinked Segments) found in human functional amyloids.
External linksNat Commun / PubMed:35896552 / PubMed Central
MethodsEM (helical sym.)
Resolution3.0 - 3.5 Å
Structure data

EMDB-14167, PDB-7qv5:
Amyloid fibril from the antimicrobial peptide uperin 3.5
Method: EM (helical sym.) / Resolution: 3.0 Å

EMDB-14168, PDB-7qv6:
Amyloid fibril from the antimicrobial peptide aurein 3.3
Method: EM (helical sym.) / Resolution: 3.5 Å

Source
  • uperoleia mjobergii (Mjoberg's toadlet)
  • ranoidea raniformis (blue-thighed treefrog)
KeywordsPROTEIN FIBRIL / antimicrobial peptide / amyloid / filament / cross-beta

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