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Title | Antigen-Triggered Logic-Gating of DNA Nanodevices. |
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Journal, issue, pages | J Am Chem Soc, Vol. 143, Issue 51, Page 21630-21636, Year 2021 |
Publish date | Dec 29, 2021 |
![]() | Wouter Engelen / Christian Sigl / Karoline Kadletz / Elena M Willner / Hendrik Dietz / ![]() |
PubMed Abstract | Synthetic nanoscale devices that reconfigure dynamically in response to physiological stimuli could offer new avenues for diagnostics and therapy. Here, we report a strategy for controlling the state ...Synthetic nanoscale devices that reconfigure dynamically in response to physiological stimuli could offer new avenues for diagnostics and therapy. Here, we report a strategy for controlling the state of DNA nanodevices based on sensing antigens with IgG antibodies. To this end, we use IgG antibodies as structural elements to kinetically trap reconfigurable DNA origami structures in metastable states. Addition of soluble antigens displace the IgGs from the objects and triggers reconfiguration. We demonstrate this mechanism by antigen-triggered disassembly of DNA origami shells for two different IgGs and their cognate antigens, and we determined the corresponding dose response curves. We also describe the logic-gated actuation of DNA objects with combinations of antigens, as demonstrated with AND-type shells that disassemble only when two different antigens are detected simultaneously. We apply our system for the antigen-triggered release of molecular payload as exemplified by the release of virus particles that we loaded into the DNA origami shells. We expect our approach to be applicable in many types of DNA nanostructures and with many other IgG-antigen combinations. |
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Methods | EM (single particle) |
Resolution | 22.7 Å |
Structure data | ![]() EMDB-13888: Antigen-Triggered Logic-Gating of DNA Nanodevices: T=1 DNA origami shell with two antidigoxigenin antibodies |
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