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TitleHow to build a ribosome from RNA fragments in Chlamydomonas mitochondria.
Journal, issue, pagesNat Commun, Vol. 12, Issue 1, Page 7176, Year 2021
Publish dateDec 9, 2021
AuthorsFlorent Waltz / Thalia Salinas-Giegé / Robert Englmeier / Herrade Meichel / Heddy Soufari / Lauriane Kuhn / Stefan Pfeffer / Friedrich Förster / Benjamin D Engel / Philippe Giegé / Laurence Drouard / Yaser Hashem /
PubMed AbstractMitochondria are the powerhouse of eukaryotic cells. They possess their own gene expression machineries where highly divergent and specialized ribosomes, named hereafter mitoribosomes, translate the ...Mitochondria are the powerhouse of eukaryotic cells. They possess their own gene expression machineries where highly divergent and specialized ribosomes, named hereafter mitoribosomes, translate the few essential messenger RNAs still encoded by mitochondrial genomes. Here, we present a biochemical and structural characterization of the mitoribosome in the model green alga Chlamydomonas reinhardtii, as well as a functional study of some of its specific components. Single particle cryo-electron microscopy resolves how the Chlamydomonas mitoribosome is assembled from 13 rRNA fragments encoded by separate non-contiguous gene pieces. Additional proteins, mainly OPR, PPR and mTERF helical repeat proteins, are found in Chlamydomonas mitoribosome, revealing the structure of an OPR protein in complex with its RNA binding partner. Targeted amiRNA silencing indicates that these ribosomal proteins are required for mitoribosome integrity. Finally, we use cryo-electron tomography to show that Chlamydomonas mitoribosomes are attached to the inner mitochondrial membrane via two contact points mediated by Chlamydomonas-specific proteins. Our study expands our understanding of mitoribosome diversity and the various strategies these specialized molecular machines adopt for membrane tethering.
External linksNat Commun / PubMed:34887394 / PubMed Central
MethodsEM (single particle) / EM (subtomogram averaging)
Resolution3.0 - 31.6 Å
Structure data

13480

7pkt

EMDB-13480, PDB-7pkt:
Large subunit of the Chlamydomonas reinhardtii mitoribosome
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-13481:
Small subunit of the Chlamydomonas mitoribosome - head focus
Method: EM (single particle) / Resolution: 4.47 Å

EMDB-13578:
C.reinhardtii mitochondrial ribosome (in situ)
Method: EM (subtomogram averaging) / Resolution: 31.6 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER / Iron–sulfur cluster

Source
  • chlamydomonas reinhardtii (plant)
KeywordsRIBOSOME / Mitochondria / mitoribosome / alga

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