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TitleSpontaneous reverse movement of mRNA-bound tRNA through the ribosome.
Journal, issue, pagesNat Struct Mol Biol, Vol. 14, Issue 4, Page 318-324, Year 2007
Publish dateMar 18, 2007
AuthorsAndrey L Konevega / Niels Fischer / Yuri P Semenkov / Holger Stark / Wolfgang Wintermeyer / Marina V Rodnina /
PubMed AbstractDuring the translocation step of protein synthesis, a complex of two transfer RNAs bound to messenger RNA (tRNA-mRNA) moves through the ribosome. The reaction is promoted by an elongation factor, ...During the translocation step of protein synthesis, a complex of two transfer RNAs bound to messenger RNA (tRNA-mRNA) moves through the ribosome. The reaction is promoted by an elongation factor, called EF-G in bacteria, which, powered by GTP hydrolysis, induces an open, unlocked conformation of the ribosome that allows for spontaneous tRNA-mRNA movement. Here we show that, in the absence of EF-G, there is spontaneous backward movement, or retrotranslocation, of two tRNAs bound to mRNA. Retrotranslocation is driven by the gain in affinity when a cognate E-site tRNA moves into the P site, which compensates the affinity loss accompanying the movement of peptidyl-tRNA from the P to the A site. These results lend support to the diffusion model of tRNA movement during translocation. In the cell, tRNA movement is biased in the forward direction by EF-G, which acts as a Brownian ratchet and prevents backward movement.
External linksNat Struct Mol Biol / PubMed:17369838
MethodsEM (single particle)
Resolution12.0 - 16.0 Å
Structure data

EMDB-1323:
Spontaneous reverse movement of mRNA-bound tRNA through the ribosome.
Method: EM (single particle) / Resolution: 16.0 Å

EMDB-1324:
Spontaneous reverse movement of mRNA-bound tRNA through the ribosome.
Method: EM (single particle) / Resolution: 12.0 Å

Source
  • Escherichia coli (E. coli)

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