+Search query
-Structure paper
Title | High-resolution model of Arabidopsis Photosystem II reveals the structural consequences of digitonin-extraction. |
---|---|
Journal, issue, pages | Sci Rep, Vol. 11, Issue 1, Page 15534, Year 2021 |
Publish date | Jul 30, 2021 |
Authors | André T Graça / Michael Hall / Karina Persson / Wolfgang P Schröder / |
PubMed Abstract | In higher plants, the photosynthetic process is performed and regulated by Photosystem II (PSII). Arabidopsis thaliana was the first higher plant with a fully sequenced genome, conferring it the ...In higher plants, the photosynthetic process is performed and regulated by Photosystem II (PSII). Arabidopsis thaliana was the first higher plant with a fully sequenced genome, conferring it the status of a model organism; nonetheless, a high-resolution structure of its Photosystem II is missing. We present the first Cryo-EM high-resolution structure of Arabidopsis PSII supercomplex with average resolution of 2.79 Å, an important model for future PSII studies. The digitonin extracted PSII complexes demonstrate the importance of: the LHG2630-lipid-headgroup in the trimerization of the light-harvesting complex II; the stabilization of the PsbJ subunit and the CP43-loop E by DGD520-lipid; the choice of detergent for the integrity of membrane protein complexes. Furthermore, our data shows at the anticipated MnCaO-site a single metal ion density as a reminiscent early stage of Photosystem II photoactivation. |
External links | Sci Rep / PubMed:34330992 / PubMed Central |
Methods | EM (single particle) |
Resolution | 2.79 Å |
Structure data | EMDB-13078, PDB-7oui: |
Chemicals | ChemComp-CHL: ChemComp-CLA: ChemComp-LHG: ChemComp-PHO: ChemComp-BCR: ChemComp-SQD: ChemComp-LMG: ChemComp-PL9: ChemComp-AJP: ChemComp-BCT: ChemComp-FE2: ChemComp-DGD: ChemComp-CA: ChemComp-CL: ChemComp-HEM: ChemComp-LUT: ChemComp-NEX: ChemComp-XAT: ChemComp-HOH: |
Source |
|
Keywords | PHOTOSYNTHESIS / Oxidoreductase / Complex |