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Structure paper

TitleStructure of ABCB1/P-Glycoprotein in the Presence of the CFTR Potentiator Ivacaftor.
Journal, issue, pagesMembranes (Basel), Vol. 11, Issue 12, Year 2021
Publish dateNov 25, 2021
AuthorsAlessandro Barbieri / Nopnithi Thonghin / Talha Shafi / Stephen M Prince / Richard F Collins / Robert C Ford /
PubMed AbstractABCB1/P-glycoprotein is an ATP binding cassette transporter that is involved in the clearance of xenobiotics, and it affects the disposition of many drugs in the body. Conformational flexibility of ...ABCB1/P-glycoprotein is an ATP binding cassette transporter that is involved in the clearance of xenobiotics, and it affects the disposition of many drugs in the body. Conformational flexibility of the protein within the membrane is an intrinsic part of its mechanism of action, but this has made structural studies challenging. Here, we have studied different conformations of P-glycoprotein simultaneously in the presence of ivacaftor, a known competitive inhibitor. In order to conduct this, we used high contrast cryo-electron microscopy imaging with a Volta phase plate. We associate the presence of ivacaftor with the appearance of an additional density in one of the conformational states detected. The additional density is in the central aqueous cavity and is associated with a wider separation of the two halves of the transporter in the inward-facing state. Conformational changes to the nucleotide-binding domains are also observed and may help to explain the stimulation of ATPase activity that occurs when transported substrate is bound in many ATP binding cassette transporters.
External linksMembranes (Basel) / PubMed:34940424 / PubMed Central
MethodsEM (single particle)
Resolution4.2 - 5.4 Å
Structure data

13059

7otg

EMDB-13059, PDB-7otg:
Structure of ABCB1/P-glycoprotein in the presence of the CFTR potentiator ivacaftor
Method: EM (single particle) / Resolution: 5.4 Å

13060

7oti

EMDB-13060, PDB-7oti:
Structure of ABCB1/P-glycoprotein in apo state
Method: EM (single particle) / Resolution: 4.2 Å

Chemicals

ChemComp-VX7:
N-(2,4-di-tert-butyl-5-hydroxyphenyl)-4-oxo-1,4-dihydroquinoline-3-carboxamide / medication*YM

Source
  • mus musculus (house mouse)
KeywordsMEMBRANE PROTEIN / ABCB1 / ABC Transporters / Membrane Proteins / Ivacaftor / MDR1 / Multidrug resistance / cryo-em

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