EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Mechanosensitive channel gating by delipidation.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 118, Issue 33, Year 2021
掲載日	2021年8月17日
著者	Vanessa Judith Flegler / Akiko Rasmussen / Karina Borbil / Lea Boten / Hsuan-Ai Chen / Hanna Deinlein / Julia Halang / Kristin Hellmanzik / Jessica Löffler / Vanessa Schmidt / Cihan Makbul / Christian Kraft / Rainer Hedrich / Tim Rasmussen / Bettina Böttcher /
PubMed 要旨	The mechanosensitive channel of small conductance (MscS) protects bacteria against hypoosmotic shock. It can sense the tension in the surrounding membrane and releases solutes if the pressure in the ...The mechanosensitive channel of small conductance (MscS) protects bacteria against hypoosmotic shock. It can sense the tension in the surrounding membrane and releases solutes if the pressure in the cell is getting too high. The membrane contacts MscS at sensor paddles, but lipids also leave the membrane and move along grooves between the paddles to reside as far as 15 Å away from the membrane in hydrophobic pockets. One sensing model suggests that a higher tension pulls lipids from the grooves back to the membrane, which triggers gating. However, it is still unclear to what degree this model accounts for sensing and what contribution the direct interaction of the membrane with the channel has. Here, we show that MscS opens when it is sufficiently delipidated by incubation with the detergent dodecyl-β-maltoside or the branched detergent lauryl maltose neopentyl glycol. After addition of detergent-solubilized lipids, it closes again. These results support the model that lipid extrusion causes gating: Lipids are slowly removed from the grooves and pockets by the incubation with detergent, which triggers opening. Addition of lipids in micelles allows lipids to migrate back into the pockets, which closes the channel even in the absence of a membrane. Based on the distribution of the aliphatic chains in the open and closed conformation, we propose that during gating, lipids leave the complex on the cytosolic leaflet at the height of highest lateral tension, while on the periplasmic side, lipids flow into gaps, which open between transmembrane helices.
リンク	Proc Natl Acad Sci U S A / PubMed:34376558 / PubMed Central
手法	EM (単粒子)
解像度	2.3 - 3.9 Å
構造データ	12996 7onj EMDB-12996, PDB-7onj: Mechanosensitive channel MscS solubilized with LMNG in open conformation 手法: EM (単粒子) / 解像度: 2.3 Å 12997 7onl EMDB-12997, PDB-7onl: Mechanosensitive channel MscS solubilized with DDM in closed conformation 手法: EM (単粒子) / 解像度: 3.9 Å 13003 7oo0 EMDB-13003, PDB-7oo0: Mechanosensitive channel MscS solubilized with DDM in open conformation 手法: EM (単粒子) / 解像度: 3.1 Å 13006 7oo6 EMDB-13006, PDB-7oo6: Mechanosensitive channel MscS solubilized with DDM in closed conformation with added lipid 手法: EM (単粒子) / 解像度: 3.1 Å 13007 7oo8 EMDB-13007, PDB-7oo8: Mechanosensitive channel MscS solubilized with LMNG in closed conformation with added lipid 手法: EM (単粒子) / 解像度: 3.7 Å 13008 7ooa EMDB-13008, PDB-7ooa: Mechanosensitive channel MscS solubilized with LMNG in open conformation with added lipid 手法: EM (単粒子) / 解像度: 2.7 Å
化合物	ChemComp-PEE: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine / DOPE, リン脂質YM / Discrete optimized protein energy ChemComp-LMT: DODECYL-BETA-D-MALTOSIDE / ドデシルβ-D-マルトシド / 可溶化剤YM ChemComp-AV0: Lauryl Maltose Neopentyl Glycol / ラウリルマルト-スネオペンチルグリコ-ル ChemComp-HOH: WATER / 水 ChemComp-PCW: 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE / O-(1-O,2-O-ジオレオイル-L-グリセロ-3-ホスホ)コリン / DOPC, リン脂質*YM
由来	escherichia coli (大腸菌) escherichia coli (strain k12) (大腸菌) escherichia coli se11 (大腸菌) Escherichia coli SE11
キーワード	MEMBRANE PROTEIN (膜タンパク質) / mechanosensitive channel / LMNG stack / delipidation / DDM / LMNG