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TitleStructure of the dodecahedral penton particle from human adenovirus type 3.
Journal, issue, pagesJ Mol Biol, Vol. 356, Issue 2, Page 510-520, Year 2006
Publish dateFeb 17, 2006
AuthorsP Fuschiotti / G Schoehn / P Fender / C M S Fabry / E A Hewat / J Chroboczek / R W H Ruigrok / J F Conway /
PubMed AbstractThe sub-viral dodecahedral particle of human adenovirus type 3, composed of the viral penton base and fiber proteins, shares an important characteristic of the entire virus: it can attach to cells ...The sub-viral dodecahedral particle of human adenovirus type 3, composed of the viral penton base and fiber proteins, shares an important characteristic of the entire virus: it can attach to cells and penetrate them. Structure determination of the fiberless dodecahedron by cryo-electron microscopy to 9 Angstroms resolution reveals tightly bound pentamer subunits, with only minimal interfaces between penton bases stabilizing the fragile dodecahedron. The internal cavity of the dodecahedron is approximately 80 Angstroms in diameter, and the interior surface is accessible to solvent through perforations of approximately 20 Angstroms diameter between the pentamer towers. We observe weak density beneath pentamers that we attribute to a penton base peptide including residues 38-48. The intact amino-terminal domain appears to interfere with pentamer-pentamer interactions and its absence by mutation or proteolysis is essential for dodecamer assembly. Differences between the 9 Angstroms dodecahedron structure and the adenovirus serotype 2 (Ad2) crystallographic model correlate closely with differences in sequence. The 3D structure of the dodecahedron including fibers at 16 Angstroms resolution reveals extra density on the top of the penton base that can be attributed to the fiber N terminus. The fiber itself exhibits striations that correlate with features of the atomic structure of the partial Ad2 fiber and that represent a repeat motif present in the amino acid sequence. These new observations offer important insights into particle assembly and stability, as well as the practicality of using the dodecahedron in targeted drug delivery. The structural work provides a sound basis for manipulating the properties of this particle and thereby enhancing its value for such therapeutic use.
External linksJ Mol Biol / PubMed:16375921
MethodsEM (single particle)
Resolution9.3 - 16.5 Å
Structure data

1178

2c9f

2c9g

EMDB-1178: Structure of the dodecahedral penton particle from human adenovirus type 3.
PDB-2c9f: THE QUASI-ATOMIC MODEL OF THE ADENOVIRUS TYPE 3 PENTON DODECAHEDRON
PDB-2c9g: THE QUASI-ATOMIC MODEL OF THE ADENOVIRUS TYPE 3 PENTON BASE DODECAHEDRON
Method: EM (single particle) / Resolution: 9.3 Å

EMDB-1179:
Structure of the dodecahedral penton particle from human adenovirus type 3.
Method: EM (single particle) / Resolution: 16.5 Å

Source
  • Human adenovirus 3
  • human adenovirus type 2
  • human adenovirus 2
KeywordsVIRUS/VIRAL PROTEIN / VIRUS-VIRAL PROTEIN COMPLEX / DODECAHEDRON / PENTON / FIBRE / LATE PROTEIN

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