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TitleThe Architecture of Inactivated SARS-CoV-2 with Postfusion Spikes Revealed by Cryo-EM and Cryo-ET.
Journal, issue, pagesStructure, Vol. 28, Issue 11, Page 1218-11224.e4, Year 2020
Publish dateNov 3, 2020
AuthorsChuang Liu / Luiza Mendonça / Yang Yang / Yuanzhu Gao / Chenguang Shen / Jiwei Liu / Tao Ni / Bin Ju / Congcong Liu / Xian Tang / Jinli Wei / Xiaomin Ma / Yanan Zhu / Weilong Liu / Shuman Xu / Yingxia Liu / Jing Yuan / Jing Wu / Zheng Liu / Zheng Zhang / Lei Liu / Peiyi Wang / Peijun Zhang /
PubMed AbstractThe ongoing global pandemic of coronavirus disease 2019 (COVID-19) resulted from the outbreak of SARS-CoV-2 in December 2019. Currently, multiple efforts are being made to rapidly develop vaccines ...The ongoing global pandemic of coronavirus disease 2019 (COVID-19) resulted from the outbreak of SARS-CoV-2 in December 2019. Currently, multiple efforts are being made to rapidly develop vaccines and treatments to fight COVID-19. Current vaccine candidates use inactivated SARS-CoV-2 viruses; therefore, it is important to understand the architecture of inactivated SARS-CoV-2. We have genetically and structurally characterized β-propiolactone-inactivated viruses from a propagated and purified clinical strain of SARS-CoV-2. We observed that the virus particles are roughly spherical or moderately pleiomorphic. Although a small fraction of prefusion spikes are found, most spikes appear nail shaped, thus resembling a postfusion state, where the S1 protein of the spike has disassociated from S2. Cryoelectron tomography and subtomogram averaging of these spikes yielded a density map that closely matches the overall structure of the SARS-CoV postfusion spike and its corresponding glycosylation site. Our findings have major implications for SARS-CoV-2 vaccine design, especially those using inactivated viruses.
External linksStructure / PubMed:33058760 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution10.7 Å
Structure data

EMDB-11627:
Subtomogram average of beta-propiolactone-treated SARS-CoV-2 post-fusion spike
Method: EM (subtomogram averaging) / Resolution: 10.7 Å

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