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-Structure paper
Title | Structure of plant photosystem I-plastocyanin complex reveals strong hydrophobic interactions. |
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Journal, issue, pages | Biochem J, Vol. 478, Issue 12, Page 2371-2384, Year 2021 |
Publish date | Jun 25, 2021 |
![]() | Ido Caspy / Mariia Fadeeva / Sebastian Kuhlgert / Anna Borovikova-Sheinker / Daniel Klaiman / Gal Masrati / Friedel Drepper / Nir Ben-Tal / Michael Hippler / Nathan Nelson / ![]() ![]() ![]() |
PubMed Abstract | Photosystem I is defined as plastocyanin-ferredoxin oxidoreductase. Taking advantage of genetic engineering, kinetic analyses and cryo-EM, our data provide novel mechanistic insights into binding and ...Photosystem I is defined as plastocyanin-ferredoxin oxidoreductase. Taking advantage of genetic engineering, kinetic analyses and cryo-EM, our data provide novel mechanistic insights into binding and electron transfer between PSI and Pc. Structural data at 2.74 Å resolution reveals strong hydrophobic interactions in the plant PSI-Pc ternary complex, leading to exclusion of water molecules from PsaA-PsaB/Pc interface once the PSI-Pc complex forms. Upon oxidation of Pc, a slight tilt of bound oxidized Pc allows water molecules to accommodate the space between Pc and PSI to drive Pc dissociation. Such a scenario is consistent with the six times larger dissociation constant of oxidized as compared with reduced Pc and mechanistically explains how this molecular machine optimized electron transfer for fast turnover. |
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Methods | EM (single particle) |
Resolution | 2.74 Å |
Structure data | EMDB-11326, PDB-6zoo: |
Chemicals | ![]() ChemComp-CL0: ![]() ChemComp-CLA: ![]() ChemComp-PQN: ![]() ChemComp-SF4: ![]() ChemComp-BCR: ![]() ChemComp-LHG: ![]() ChemComp-LMT: ![]() ChemComp-LMG: ![]() ChemComp-CA: ![]() ChemComp-DGD: ![]() ChemComp-LUT: ![]() ChemComp-CHL: ![]() ChemComp-XAT: ![]() ChemComp-3PH: ![]() ChemComp-CU: ![]() ChemComp-HOH: |
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![]() | PHOTOSYNTHESIS / Photosystem I / membrane protein |