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TitleStructural heterogeneity of a human norovirus vaccine candidate.
Journal, issue, pagesVirology, Vol. 553, Page 23-34, Year 2021
Publish dateJan 15, 2021
AuthorsJessica M Devant / Grant S Hansman /
PubMed AbstractHuman norovirus virus-like particles (VLPs) are assumed to be morphologically and antigenically similar to virion particles. The norovirus virion is assembled from 180 copies of the capsid protein ...Human norovirus virus-like particles (VLPs) are assumed to be morphologically and antigenically similar to virion particles. The norovirus virion is assembled from 180 copies of the capsid protein (VP1) and exhibits T = 3 icosahedral symmetry. In this study, we showed that the vaccine candidate GII.4c VP1 formed T = 1 and T = 3 VLPs, but mainly assembled into T = 4 icosahedral particles that were composed of 240 VP1 copies. In contrast, another clinically important genotype, GII.17, almost exclusively folded into T = 3 VLPs. Interestingly, the GII.4c T = 1 particles had higher binding capacities to norovirus-specific Nanobodies than to GII.4c T = 3 and T = 4 particles. Our data indicated that the occluded Nanobody-binding epitopes on the T = 1 particles were more accessible compared to the larger T = 3 and T = 4 particles. Overall, this new data revealed that GII.4c VLPs had a preference for forming the T = 4 icosahedral symmetry and future studies with varied sized norovirus VLPs should take caution when examining antigenicity.
External linksVirology / PubMed:33202318
MethodsEM (single particle)
Resolution4.17 - 8.05 Å
Structure data

EMDB-10755:
Cryo-EM structure of norovirus GII.4c VLP with T=4 icosahedral symmetry
Method: EM (single particle) / Resolution: 4.17 Å

EMDB-10756:
Cryo-EM structure of norovirus GII.4c VLPs with T=3 icosahedral symmetry
Method: EM (single particle) / Resolution: 8.05 Å

EMDB-10757:
Cryo-EM structure of norovirus GI.4c VLPs with T=1 icosahedral symmetry
Method: EM (single particle) / Resolution: 4.2 Å

EMDB-10758:
Cryo-EM structure of norovirus GII.4c VLPs expressed in HEK-293T cells with T=4 icosahedral symmetry
Method: EM (single particle) / Resolution: 4.45 Å

EMDB-10759:
Cryo-EM structure of norovirus GII.17 VLPs
Method: EM (single particle) / Resolution: 5.71 Å

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