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TitleThree-dimensional structure of a pre-catalytic human spliceosomal complex B.
Journal, issue, pagesNat Struct Mol Biol, Vol. 11, Issue 5, Page 463-468, Year 2004
Publish dateApr 18, 2004
AuthorsDaniel Boehringer / Evgeny M Makarov / Bjoern Sander / Olga V Makarova / Berthold Kastner / Reinhard Lührmann / Holger Stark /
PubMed AbstractMajor structural changes occur in the spliceosome during its transition from the fully assembled complex B to the catalytically activated spliceosome. To understand the rearrangement, it is necessary ...Major structural changes occur in the spliceosome during its transition from the fully assembled complex B to the catalytically activated spliceosome. To understand the rearrangement, it is necessary to know the detailed three-dimensional structures of these complexes. Here, we have immunoaffinity-purified human spliceosomes (designated B Delta U1) at a stage after U4/U6.U5 tri-snRNP integration but before activation, and have determined the three-dimensional structure of B Delta U1 by single-particle electron cryomicroscopy at a resolution of approximately 40 A. The overall size of the complex is about 370 x 270 x 170 A. The three-dimensional structure features a roughly triangular body linked to a head domain in variable orientations. The body is very similar in size and shape to the isolated U4/U6.U5 tri-snRNP. This provides initial insight into the structural organization of complex B.
External linksNat Struct Mol Biol / PubMed:15098019
MethodsEM (single particle)
Resolution40.0 Å
Structure data

EMDB-1066:
Three-dimensional structure of a pre-catalytic human spliceosomal complex B.
Method: EM (single particle) / Resolution: 40.0 Å

Source
  • Homo sapiens (human)

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