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TitleMechanism of effector capture and delivery by the type IV secretion system from Legionella pneumophila.
Journal, issue, pagesNat Commun, Vol. 11, Issue 1, Page 2864, Year 2020
Publish dateJun 8, 2020
AuthorsAmit Meir / Kevin Macé / Natalya Lukoyanova / David Chetrit / Manuela K Hospenthal / Adam Redzej / Craig Roy / Gabriel Waksman /
PubMed AbstractLegionella pneumophila is a bacterial pathogen that utilises a Type IV secretion (T4S) system to inject effector proteins into human macrophages. Essential to the recruitment and delivery of ...Legionella pneumophila is a bacterial pathogen that utilises a Type IV secretion (T4S) system to inject effector proteins into human macrophages. Essential to the recruitment and delivery of effectors to the T4S machinery is the membrane-embedded T4 coupling complex (T4CC). Here, we purify an intact T4CC from the Legionella membrane. It contains the DotL ATPase, the DotM and DotN proteins, the chaperone module IcmSW, and two previously uncharacterised proteins, DotY and DotZ. The atomic resolution structure reveals a DotLMNYZ hetero-pentameric core from which the flexible IcmSW module protrudes. Six of these hetero-pentameric complexes may assemble into a 1.6-MDa hexameric nanomachine, forming an inner membrane channel for effectors to pass through. Analysis of multiple cryo EM maps, further modelling and mutagenesis provide working models for the mechanism for binding and delivery of two essential classes of Legionella effectors, depending on IcmSW or DotM, respectively.
External linksNat Commun / PubMed:32513920 / PubMed Central
MethodsEM (single particle)
Resolution3.7 Å
Structure data

10350

6sz9

EMDB-10350, PDB-6sz9:
Type IV Coupling Complex (T4CC) from L. pneumophila.
Method: EM (single particle) / Resolution: 3.7 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • legionella pneumophila (bacteria)
KeywordsMEMBRANE PROTEIN / Protein Complex / Secretion / Secretion systems / Gram-negative bacteria / type 4 secretion system / T4SS / coupling protein / T4CC / DotL / DotM / DotN / DotY / DotZ

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