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TitleCombining Transient Expression and Cryo-EM to Obtain High-Resolution Structures of Luteovirid Particles.
Journal, issue, pagesStructure, Vol. 27, Issue 12, Page 1761-11770.e3, Year 2019
Publish dateDec 3, 2019
AuthorsMatthew J Byrne / John F C Steele / Emma L Hesketh / Miriam Walden / Rebecca F Thompson / George P Lomonossoff / Neil A Ranson /
PubMed AbstractThe Luteoviridae are pathogenic plant viruses responsible for significant crop losses worldwide. They infect a wide range of food crops, including cereals, legumes, cucurbits, sugar beet, sugarcane, ...The Luteoviridae are pathogenic plant viruses responsible for significant crop losses worldwide. They infect a wide range of food crops, including cereals, legumes, cucurbits, sugar beet, sugarcane, and potato and, as such, are a major threat to global food security. Viral replication is strictly limited to the plant vasculature, and this phloem limitation, coupled with the need for aphid transmission of virus particles, has made it difficult to generate virus in the quantities needed for high-resolution structural studies. Here, we exploit recent advances in heterologous expression in plants to produce sufficient quantities of virus-like particles for structural studies. We have determined their structures to high resolution by cryoelectron microscopy, providing the molecular-level insight required to rationally interrogate luteovirid capsid formation and aphid transmission, thereby providing a platform for the development of preventive agrochemicals for this important family of plant viruses.
External linksStructure / PubMed:31611039 / PubMed Central
MethodsEM (single particle)
Resolution3.0 - 3.3 Å
Structure data

EMDB-10142, PDB-6scl:
Cryo-EM Structure of Barley Yellow Dwarf Virus VLP
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-10144, PDB-6sco:
Cryo-EM Structure of Potato Leaf Roll Virus VLP
Method: EM (single particle) / Resolution: 3.3 Å

Source
  • barley yellow dwarf virus
  • potato leafroll virus
KeywordsVIRUS LIKE PARTICLE / Luteovirus / VLP / Capsid. / Polerovirus

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