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TitleStructural models of intrinsically disordered and calcium-bound folded states of a protein adapted for secretion.
Journal, issue, pagesSci Rep, Vol. 5, Page 14223, Year 2015
Publish dateSep 16, 2015
AuthorsDarragh P O'Brien / Belen Hernandez / Dominique Durand / Véronique Hourdel / Ana-Cristina Sotomayor-Pérez / Patrice Vachette / Mahmoud Ghomi / Julia Chamot-Rooke / Daniel Ladant / Sébastien Brier / Alexandre Chenal /
PubMed AbstractMany Gram-negative bacteria use Type I secretion systems, T1SS, to secrete virulence factors that contain calcium-binding Repeat-in-ToXin (RTX) motifs. Here, we present structural models of an RTX ...Many Gram-negative bacteria use Type I secretion systems, T1SS, to secrete virulence factors that contain calcium-binding Repeat-in-ToXin (RTX) motifs. Here, we present structural models of an RTX protein, RD, in both its intrinsically disordered calcium-free Apo-state and its folded calcium-bound Holo-state. Apo-RD behaves as a disordered polymer chain comprising several statistical elements that exhibit local rigidity with residual secondary structure. Holo-RD is a folded multi-domain protein with an anisometric shape. RTX motifs thus appear remarkably adapted to the structural and mechanistic constraints of the secretion process. In the low calcium environment of the bacterial cytosol, Apo-RD is an elongated disordered coil appropriately sized for transport through the narrow secretion machinery. The progressive folding of Holo-RD in the extracellular calcium-rich environment as it emerges form the T1SS may then favor its unidirectional export through the secretory channel. This process is relevant for hundreds of bacterial species producing virulent RTX proteins.
External linksSci Rep / PubMed:26374675 / PubMed Central
MethodsSAS (X-ray synchrotron)
Structure data

SASDGM5:
Holo-RD domain of B. Pertussis Adenylate Cyclase Toxin (CyaA)
Method: SAXS/SANS

SASDGN5:
apo-RD domain of B. Pertussis Adenylate Cyclase Toxin (CyaA)
Method: SAXS/SANS

Source
  • Bordetella pertussis (strain tohama i / atcc baa-589 / nctc 13251) (bacteria)

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