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-Structure paper
| タイトル | Structural basis of a GatC ortholog transporter in the bacterial phosphotransferase system. |
|---|---|
| ジャーナル・号・ページ | FEBS Lett, Vol. 599, Issue 16, Page 2377-2387, Year 2025 |
| 掲載日 | 2025年8月5日 |
著者 | Yutaro S Takahashi / Hidetaka Kohga / Min Fey Chek / Kotomi Yamamoto / Jun F Takahashi / Hideki Shigematsu / Yoshiki Tanaka / Muneyoshi Ichikawa / Ryoji Miyazaki / Toshio Hakoshima / Tomoya Tsukazaki / ![]() |
| PubMed 要旨 | The bacterial phosphotransferase system (PTS) mediates the uptake of specific carbohydrates via IIC transporters. Here, we report the crystal and cryo-electron microscopy (cryo-EM) structures of ...The bacterial phosphotransferase system (PTS) mediates the uptake of specific carbohydrates via IIC transporters. Here, we report the crystal and cryo-electron microscopy (cryo-EM) structures of Leminorella grimontii galactitol-specific PTS enzyme IIC component (LgGatC), which is implicated in D-xylose uptake and belongs to the ascorbate-galactitol (AG) superfamily of IIC proteins. These structures, determined in the presence and absence of D-xylose, capture the transporter in an outward-facing conformation. A homology model of an inward-facing state, constructed based on these structures, supports an elevator-like transport mechanism. These findings provide structural insights into substrate recognition by GatC and offer a framework for understanding sugar transport in PTS IIC proteins. |
リンク | FEBS Lett / PubMed:40878824 |
| 手法 | EM (単粒子) / X線回折 |
| 解像度 | 2.7 - 3.74 Å |
| 構造データ | EMDB-63950, PDB-9u82: EMDB-63951, PDB-9u84: ![]() PDB-9u8e: ![]() PDB-9u8h: |
| 化合物 | ![]() ChemComp-OLC: |
| 由来 |
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キーワード | TRANSPORT PROTEIN / PTS / D-xylose / transporter / xylose |
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leminorella grimontii (バクテリア)
キーワード