EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures of transcription complexes reveal how ToxR and TcpP recruit the RNA polymerase and activate virulence genes.
ジャーナル・号・ページ	Sci Adv, Vol. 12, Issue 3, Page eadx9680, Year 2026
掲載日	2026年1月16日
著者	Adrià Alcaide-Jiménez / Albert Canals / Florence Baudin / Cristina Machón / Montserrat Fàbrega-Ferrer / Olga Bantysh / Rosa Pérez-Luque / Brice Murciano / Ali A Mohammad / Michael J Rowse / Joseph M Ferracciolo / Eric S Krukonis / Christoph W Müller / Miquel Coll /
PubMed 要旨	Activation of virulence in , the etiological agent of cholera disease, is mediated by two transmembrane one-component signal-transduction proteins, ToxR and TcpP, which are also transcription factors. ...Activation of virulence in , the etiological agent of cholera disease, is mediated by two transmembrane one-component signal-transduction proteins, ToxR and TcpP, which are also transcription factors. Using cryo-electron microscopy, we have solved five structures of the and transcription activation complexes, including the RNA polymerase (RNAP) holoenzyme, promoter DNAs, transcribed RNA, and their corresponding transcription factors, ToxR or TcpP and ToxR-TcpP, respectively. Activation is achieved through the interaction of ToxR or TcpP with the α-C-terminal repeat domain of RNAP where a single residue of the activator, a phenylalanine, appears to be the most critical contact, as confirmed by mutagenesis. No interactions of the transcription factors were observed with other subunits of the RNAP, i.e., the σ subunit as it occurs in the structurally related PhoB family of two-component transcription factors. The structures, and their comparison with our previously solved DNA promoter-ToxR x-ray structures, unveil the molecular mechanism of cholera virulence gene activation.
リンク	Sci Adv / PubMed:41533787 / PubMed Central
手法	EM (単粒子)
解像度	2.6 - 7.2 Å
構造データ	51273 9gdo EMDB-51273, PDB-9gdo: Cryo-EM structure of Vibrio cholerae RNA polymerase holoenzyme bound to an ompU promoter DNA fragment 手法: EM (単粒子) / 解像度: 3.0 Å 51274 9gdp EMDB-51274, PDB-9gdp: Cryo-EM structure of Vibrio cholerae RNA polymerase holoenzyme bound to an ompU promoter DNA fragment and 5-mer RNA 手法: EM (単粒子) / 解像度: 2.6 Å 51275 9gdq EMDB-51275, PDB-9gdq: Cryo-EM structure of Vibrio cholerae RNA polymerase Transcription Activation Complex with ToxR transcription factor and ompU promoter DNA 手法: EM (単粒子) / 解像度: 3.1 Å 51276 9gdr EMDB-51276, PDB-9gdr: Cryo-EM structure of Vibrio cholerae RNA polymerase Transcription Activation Complex with TcpP transcription factor and a toxT promoter DNA fragment 手法: EM (単粒子) / 解像度: 2.7 Å 51277 9gds EMDB-51277, PDB-9gds: Cryo-EM structure of Vibrio cholerae RNA polymerase Transcription Activation Complex with ToxR and TcpP transcription factors and a toxT promoter DNA fragment 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-51278: Cryo-EM structure of Vibrio cholerae RNA polymerase dimer with ToxR and TcpP transcription factors and a toxT promoter DNA fragment 手法: EM (単粒子) / 解像度: 7.2 Å EMDB-51774: Consensus map of the cryo-EM structure of Vibrio cholerae RNA polymerase Transcription Activation Complex with ToxR transcription factor and bound to an ompU promoter DNA fragment 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-51775: Focused map #1 of the cryo-EM structure of Vibrio cholerae RNA polymerase Transcription Activation Complex with ToxR transcription factor and bound to an ompU promoter DNA fragment 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-51776: Focused map #2 of the cryo-EM structure of Vibrio cholerae RNA polymerase Transcription Activation Complex with ToxR transcription factor and bound to an ompU promoter DNA fragment 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-51948: Consensus map of the cryo-EM structure of Vibrio cholerae RNA polymerase Transcription Activation Complex with TcpP transcription factor and bound to a toxT promoter DNA fragment 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-51949: Focused map #1 of the cryo-EM structure of Vibrio cholerae RNA polymerase Transcription Activation Complex with TcpP transcription factor and bound to a toxT promoter DNA fragment 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-51950: Focused map #2 of the cryo-EM structure of Vibrio cholerae RNA polymerase Transcription Activation Complex with TcpP transcription factor and bound to a toxT promoter DNA fragment 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-51955: Consensus map of the cryo-EM structure of Vibrio cholerae RNA polymerase Transcription Activation Complex with ToxR and TcpP transcription factors and bound to a toxT promoter DNA fragment 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-51956: Focused map #1 of the cryo-EM structure of Vibrio cholerae RNA polymerase Transcription Activation Complex with ToxR and TcpP transcription factors and bound to a toxT promoter DNA fragment 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-51958: Focused map #2 of the cryo-EM structure of Vibrio cholerae RNA polymerase Transcription Activation Complex with ToxR and TcpP transcription factors and bound to a toxT promoter DNA fragment 手法: EM (単粒子) / 解像度: 2.9 Å
化合物	ChemComp-MG: Unknown entry ChemComp-ZN: Unknown entry
由来	vibrio cholerae o395 (コレラ菌) vibrio cholerae o1 biovar el tor str. n16961 (コレラ菌)
キーワード	TRANSCRIPTION / RNA polymerase / Open promoter complex / ompU / Vibrio cholerae / Transcription Activation Complex / toxT / TcpP / ToxR