EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Lipid interactions and gating hysteresis suggest a physiological role for mechanosensitive channel YnaI.
ジャーナル・号・ページ	Nat Commun, Vol. 16, Issue 1, Page 7472, Year 2025
掲載日	2025年8月12日
著者	Nathan Will / Giorgos Hiotis / Yoshitaka Nakayama / Gabriella Angiulli / Zijing Zhou / Charles D Cox / Boris Martinac / Thomas Walz /
PubMed 要旨	YnaI is a member of the family of bacterial MscS (mechanosensitive channel of small conductance)-like channels. Channel gating upon hypoosmotic stress and the role of lipids in this process have been ...YnaI is a member of the family of bacterial MscS (mechanosensitive channel of small conductance)-like channels. Channel gating upon hypoosmotic stress and the role of lipids in this process have been extensively studied for MscS, but are less well understood for YnaI, which features two additional transmembrane helices. Here, we combined cryogenic electron microscopy, molecular dynamics simulations and patch-clamp electrophysiology to advance our understanding of YnaI. The two additional helices move the lipid-filled hydrophobic pockets in YnaI further away from the lipid bilayer and change the function of the pocket lipids from being a critical gating element in MscS to being more of a structural element in YnaI. Unlike MscS, YnaI shows pronounced gating hysteresis and remains open to a substantially lower membrane tension than is needed to initially open the channel. Thus, at near-lytic membrane tension, both MscL and YnaI will open, but while MscL has a large pore and must close quickly to minimize loss of essential metabolites, YnaI only conducts ions and can thus remain open for longer to continue to facilitate pressure equilibration across the membrane.
リンク	Nat Commun / PubMed:40796571 / PubMed Central
手法	EM (単粒子)
解像度	2.3 - 3.0 Å
構造データ	47547 9e62 EMDB-47547, PDB-9e62: Cryo-EM structure of mechanosensitive channel YnaI in DOPC nanodiscs 手法: EM (単粒子) / 解像度: 2.5 Å 47548 9e63 EMDB-47548, PDB-9e63: Cryo-EM structure of mechanosensitive channel YnaI in DOPC nanodiscs treated with beta-cyclodextrin 手法: EM (単粒子) / 解像度: 2.3 Å 47549 9e64 EMDB-47549, PDB-9e64: Cryo-EM structure of mechanosensitive channel YnaI in DOPC nanodiscs treated with lysophosphatidylcholine 手法: EM (単粒子) / 解像度: 3.0 Å 47550 9e65 EMDB-47550, PDB-9e65: Cryo-EM structure of mechanosensitive channel YnaI A155V mutant in conformation 1 手法: EM (単粒子) / 解像度: 2.8 Å 47551 9e66 EMDB-47551, PDB-9e66: Cryo-EM structure of mechanosensitive channel YnaI A155V mutant in conformation 2 手法: EM (単粒子) / 解像度: 2.8 Å 47552 9e67 EMDB-47552, PDB-9e67: Cryo-EM structure of mechanosensitive channel YnaI in DDPC nanodiscs 手法: EM (単粒子) / 解像度: 2.3 Å 47553 9e68 EMDB-47553, PDB-9e68: Cryo-EM structure of MscS/YnaI chimera in DOPC nanodiscs 手法: EM (単粒子) / 解像度: 2.5 Å
化合物	ChemComp-PTY: PHOSPHATIDYLETHANOLAMINE / リン脂質*YM
由来	escherichia coli (大腸菌)
キーワード	MEMBRANE PROTEIN / Mechanosensitive