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-Structure paper
| タイトル | Characterization of the OMP biogenesis machinery in Fusobacterium nucleatum. |
|---|---|
| ジャーナル・号・ページ | Structure, Vol. 33, Issue 11, Page 1878-11892.e5, Year 2025 |
| 掲載日 | 2025年11月6日 |
著者 | Claire Overly Cottom / Eva Heinz / Satchal Erramilli / Anthony Kossiakoff / Daniel J Slade / Nicholas Noinaj / ![]() |
| PubMed 要旨 | F. nucleatum is a Gram-negative bacteria that causes oral infections and is linked to colorectal cancer. Pathogenicity relies on a type of β-barrel outer membrane protein (OMP) called an ...F. nucleatum is a Gram-negative bacteria that causes oral infections and is linked to colorectal cancer. Pathogenicity relies on a type of β-barrel outer membrane protein (OMP) called an autotransporter. The biogenesis of OMPs is typically mediated by the barrel assembly machinery (BAM) complex. In this study, we investigate the evolution, composition, and structure of the OMP biogenesis machinery in F. nucleatum. Our bioinformatics and proteomics analyses indicate that OMP biogenesis in F. nucleatum is mediated solely by the core component BamA. The structure of FnBamA highlights distinct features, including four POTRA domains and a C-terminal 16-stranded β-barrel domain observed as an inverted dimer. FnBamA represents the original composition of the assembly machinery, and a duplication event that resulted in BamA and TamA occurred after the split of other lineages, including the Proteobacteria, from the Fusobacteria. FnBamA, therefore, likely serves a singular role in the biogenesis of all OMPs. |
リンク | Structure / PubMed:40897170 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 3.87 Å |
| 構造データ | EMDB-45442, PDB-9ccg: |
| 由来 |
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キーワード | MEMBRANE PROTEIN / proteomics / protein folding / structural biology |
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fusobacterium nucleatum (バクテリア)
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