EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structures of Thogoto virus polymerase reveal unique RNA transcription and replication mechanisms among orthomyxoviruses.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 4620, Year 2024
掲載日	2024年5月30日
著者	Lu Xue / Tiancai Chang / Zimu Li / Chenchen Wang / Heyu Zhao / Mei Li / Peng Tang / Xin Wen / Mengmeng Yu / Jiqin Wu / Xichen Bao / Xiaojun Wang / Peng Gong / Jun He / Xinwen Chen / Xiaoli Xiong /
PubMed 要旨	Influenza viruses and thogotoviruses account for most recognized orthomyxoviruses. Thogotoviruses, exemplified by Thogoto virus (THOV), are capable of infecting humans using ticks as vectors. THOV ...Influenza viruses and thogotoviruses account for most recognized orthomyxoviruses. Thogotoviruses, exemplified by Thogoto virus (THOV), are capable of infecting humans using ticks as vectors. THOV transcribes mRNA without the extraneous 5' end sequences derived from cap-snatching in influenza virus mRNA. Here, we report cryo-EM structures to characterize THOV polymerase RNA synthesis initiation and elongation. The structures demonstrate that THOV RNA transcription and replication are able to start with short dinucleotide primers and that the polymerase cap-snatching machinery is likely non-functional. Triggered by RNA synthesis, asymmetric THOV polymerase dimers can form without the involvement of host factors. We confirm that, distinctive from influenza viruses, THOV-polymerase RNA synthesis is weakly dependent of the host factors ANP32A/B/E in human cells. This study demonstrates varied mechanisms in RNA synthesis and host factor utilization among orthomyxoviruses, providing insights into the mechanisms behind thogotoviruses' broad-infectivity range.
リンク	Nat Commun / PubMed:38816392 / PubMed Central
手法	EM (単粒子)
解像度	2.3 - 3.16 Å
構造データ	39838 8z85 EMDB-39838, PDB-8z85: Cryo-EM structure of Thogoto virus polymerase in transcription pre-initiation conformation 1 手法: EM (単粒子) / 解像度: 2.3 Å 39848 8z8j EMDB-39848, PDB-8z8j: Cryo-EM structure of Thogoto virus polymerase in transcription pre-initiation conformation 2 手法: EM (単粒子) / 解像度: 3.16 Å 39849 8z8n EMDB-39849, PDB-8z8n: Cryo-EM structure of Thogoto virus polymerase in transcription pre-initiation conformation 3 手法: EM (単粒子) / 解像度: 2.79 Å 39850 8z8x EMDB-39850, PDB-8z8x: Cryo-EM structure of Thogoto virus polymerase in a transcription initiation conformation 手法: EM (単粒子) / 解像度: 3.06 Å 39852 8z90 EMDB-39852, PDB-8z90: Cryo-EM structure of Thogoto virus polymerase in transcription initiation conformation 2 手法: EM (単粒子) / 解像度: 2.87 Å 39855 8z97 EMDB-39855, PDB-8z97: Cryo-EM structure of Thogoto virus polymerase in a transcription elongation conformation 手法: EM (単粒子) / 解像度: 2.65 Å 39856 8z98 EMDB-39856, PDB-8z98: Cryo-EM structure of Thogoto virus polymerase in a transcription reception conformation 手法: EM (単粒子) / 解像度: 2.52 Å 39862 8z9h EMDB-39862, PDB-8z9h: Cryo-EM structure of Thogoto virus polymerase in a transcription elongation-reception conformation 手法: EM (単粒子) / 解像度: 2.7 Å 39867 8z9q EMDB-39867, PDB-8z9q: Cryo-EM structure of Thogoto virus polymerase in a replication reception conformation 手法: EM (単粒子) / 解像度: 2.33 Å 39868 8z9r EMDB-39868, PDB-8z9r: Cryo-EM structure of Thogoto virus polymerase in a replication elongation-reception conformation 手法: EM (単粒子) / 解像度: 2.58 Å
化合物	ChemComp-G2P: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMPCPP / GMP-CPP, エネルギー貯蔵分子類似体YM ChemComp-V9G*: 7-METHYL-GUANOSINE-5'-TRIPHOSPHATE-5'-(2'-O-METHYL)-ADENOSINE
由来	thogoto virus (isolate siar 126) (ウイルス)
キーワード	TRANSCRIPTION / RNA virus Polymerase / RNA polymerase