EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis and synergism of ATP and Na activation in bacterial K uptake system KtrAB.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 3850, Year 2024
掲載日	2024年5月8日
著者	Wesley Tien Chiang / Yao-Kai Chang / Wei-Han Hui / Shu-Wei Chang / Chen-Yi Liao / Yi-Chuan Chang / Chun-Jung Chen / Wei-Chen Wang / Chien-Chen Lai / Chun-Hsiung Wang / Siou-Ying Luo / Ya-Ping Huang / Shan-Ho Chou / Tzyy-Leng Horng / Ming-Hon Hou / Stephen P Muench / Ren-Shiang Chen / Ming-Daw Tsai / Nien-Jen Hu /
PubMed 要旨	The K uptake system KtrAB is essential for bacterial survival in low K environments. The activity of KtrAB is regulated by nucleotides and Na. Previous studies proposed a putative gating mechanism of ...The K uptake system KtrAB is essential for bacterial survival in low K environments. The activity of KtrAB is regulated by nucleotides and Na. Previous studies proposed a putative gating mechanism of KtrB regulated by KtrA upon binding to ATP or ADP. However, how Na activates KtrAB and the Na binding site remain unknown. Here we present the cryo-EM structures of ATP- and ADP-bound KtrAB from Bacillus subtilis (BsKtrAB) both solved at 2.8 Å. A cryo-EM density at the intra-dimer interface of ATP-KtrA was identified as Na, as supported by X-ray crystallography and ICP-MS. Thermostability assays and functional studies demonstrated that Na binding stabilizes the ATP-bound BsKtrAB complex and enhances its K flux activity. Comparing ATP- and ADP-BsKtrAB structures suggests that BsKtrB Arg417 and Phe91 serve as a channel gate. The synergism of ATP and Na in activating BsKtrAB is likely applicable to Na-activated K channels in central nervous system.
リンク	Nat Commun / PubMed:38719864 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.48 - 3.1 Å
構造データ	36800 8k1s EMDB-36800, PDB-8k1s: Potassium transporter KtrAB from Bacillus subtilis in ADP-bound state 手法: EM (単粒子) / 解像度: 2.83 Å EMDB-36801: Potassium transporter KtrAB from Bacillus subtilis in ADP-bound state, focused refined on KtrA octamer 手法: EM (単粒子) / 解像度: 2.84 Å EMDB-36802: Potassium transporter KtrAB from Bacillus subtilis in ADP-bound state, focused refined on KtrB dimer 手法: EM (単粒子) / 解像度: 2.86 Å 36803 8k1t EMDB-36803, PDB-8k1t: Potassium transporter KtrAB from Bacillus subtilis in ATP-bound state with addition of MgCl2 手法: EM (単粒子) / 解像度: 2.48 Å 36804 8k1u EMDB-36804, PDB-8k1u: Potassium transporter KtrAB from Bacillus subtilis in ATP-bound state with addition of EDTA and EGTA 手法: EM (単粒子) / 解像度: 2.82 Å 38477 8xmh EMDB-38477, PDB-8xmh: Potassium transporter KtrAB from Bacillus subtilis in ATP-bound state with addition of EDTA and EGTA, vertical C2 symmetry axis 手法: EM (単粒子) / 解像度: 2.85 Å 38478 8xmi EMDB-38478, PDB-8xmi: Potassium transporter KtrAB from Bacillus subtilis in ATP-bound state with addition of EDTA and EGTA, C1 symmetry 手法: EM (単粒子) / 解像度: 3.0 Å PDB-8k16: KtrA bound with ATP and thallium 手法: X-RAY DIFFRACTION / 解像度: 3.1 Å PDB-8k1k: KtrA bound with ATP and sodium 手法: X-RAY DIFFRACTION / 解像度: 3.0 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-TL: THALLIUM (I) ION ChemComp-NA: Unknown entry / ナトリウムカチオン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-K: Unknown entry / カリウムカチオン
由来	bacillus subtilis (枯草菌)
キーワード	METAL TRANSPORT / potassium channel RCK domain / METAL BINDING PROTEIN / Potassium channel / TRANSPORT PROTEIN / KtrAB / RCK / potassium / transporter