EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Growth Process of Fe-O Nanoclusters with Different Sizes Biosynthesized by Protein Nanocages.
ジャーナル・号・ページ	J Am Chem Soc, Vol. 146, Issue 17, Page 11657-11668, Year 2024
掲載日	2024年5月1日
著者	Wenming Wang / Hongfang Xi / Dan Fu / Danyang Ma / Wenjun Gong / Yaqin Zhao / Xiaomei Li / Lijie Wu / Yu Guo / Guanghua Zhao / Hongfei Wang /
PubMed 要旨	All protein-directed syntheses of metal nanoclusters (NCs) and nanoparticles (NPs) have attracted considerable attention because protein scaffolds provide a unique metal coordination environment and ...All protein-directed syntheses of metal nanoclusters (NCs) and nanoparticles (NPs) have attracted considerable attention because protein scaffolds provide a unique metal coordination environment and can adjust the shape and morphology of NCs and NPs. However, the detailed formation mechanisms of NCs or NPs directed by protein templates remain unclear. In this study, by taking advantage of the ferritin nanocage as a biotemplate to monitor the growth of Fe-O NCs as a function of time, we synthesized a series of iron NCs with different sizes and shapes and subsequently solved their corresponding three-dimensional atomic-scale structures by X-ray protein crystallography and cryo-electron microscopy. The time-dependent structure analyses revealed the growth process of these Fe-O NCs with the 4-fold channel of ferritin as nucleation sites. To our knowledge, the newly biosynthesized FeOGlu represents the largest Fe-O NCs with a definite atomic structure. This study contributes to our understanding of the formation mechanism of iron NCs and provides an effective method for metal NC synthesis.
リンク	J Am Chem Soc / PubMed:38641862
手法	EM (単粒子) / X線回折
解像度	2.059 - 3.2 Å
構造データ	37754 8wqu EMDB-37754, PDB-8wqu: Fe-O nanocluster of form-IX in the 4-fold channel of Ureaplasma diversum ferritin 手法: EM (単粒子) / 解像度: 2.8 Å 37755 8wqv EMDB-37755, PDB-8wqv: Fe-O nanocluster of form-VIII in the 4-fold channel of Ureaplasma diversum ferritin 手法: EM (単粒子) / 解像度: 2.7 Å 37757 8wqx EMDB-37757, PDB-8wqx: Fe-O nanocluster of form-X in the 4-fold channel of Ureaplasma diversum ferritin 手法: EM (単粒子) / 解像度: 3.2 Å 37758 8wqy EMDB-37758, PDB-8wqy: Fe-O nanocluster of form-XI in the 4-fold channel of Ureaplasma diversum ferritin 手法: EM (単粒子) / 解像度: 3.0 Å 37759 8wr0 EMDB-37759, PDB-8wr0: Fe-O nanocluster of form-XII in the 4-fold channel of Ureaplasma diversum ferritin 手法: EM (単粒子) / 解像度: 3.1 Å PDB-8w6m: Native strucutre of ferritin from Ureaplasma diversum 手法: X-RAY DIFFRACTION / 解像度: 2.501 Å PDB-8w6q: ferritin from Ureaplasma diversum soaking in Fe2+ solution for 0 min 手法: X-RAY DIFFRACTION / 解像度: 2.101 Å PDB-8w6s: Ferritin from Ureaplasma diversum soaking in Fe2+ solution for 2 min 手法: X-RAY DIFFRACTION / 解像度: 2.099 Å PDB-8w6u: Ferritin from Ureaplasma diversum soaking in Fe2+ solution for 5 min 手法: X-RAY DIFFRACTION / 解像度: 2.099 Å PDB-8w6y: Ferritin from Ureaplasma diversum soaking in Fe2+ solution for 10 min 手法: X-RAY DIFFRACTION / 解像度: 2.502 Å PDB-8w73: Fe-O nanocluster of form-I in the 4-fold channel of Ureaplasma diversum ferritin 手法: X-RAY DIFFRACTION / 解像度: 2.601 Å PDB-8w74: Fe-O nanocluster of form-II in the 4-fold channel of Ureaplasma diversum ferritin 手法: X-RAY DIFFRACTION / 解像度: 2.5 Å PDB-8w79: Fe-O nanocluster of form-III in the 4-fold channel of Ureaplasma diversum ferritin 手法: X-RAY DIFFRACTION / 解像度: 2.697 Å PDB-8w7b: Fe-O nanocluster of form-IV in the 4-fold channel of Ureaplasma diversum ferritin 手法: X-RAY DIFFRACTION / 解像度: 2.7 Å PDB-8w7o: Fe-O nanocluster of form-V in the 4-fold channel of Ureaplasma diversum ferritin 手法: X-RAY DIFFRACTION / 解像度: 2.399 Å PDB-8w7q: Fe-O nanocluster of form-VI in the 4-fold channel of Ureaplasma diversum ferritin 手法: X-RAY DIFFRACTION / 解像度: 2.1 Å PDB-8w7t: Fe-O nanocluster of form-VII in the 4-fold channel of Ureaplasma diversum ferritin 手法: X-RAY DIFFRACTION / 解像度: 2.499 Å PDB-8w7u: Mutant of ferritin from Ureaplasma diversum (Udif-E164A-E168A) without soaking 手法: X-RAY DIFFRACTION / 解像度: 2.502 Å PDB-8w7v: Udif-E164A-E168A soaking in Fe2+ solution for 50 minutes 手法: X-RAY DIFFRACTION / 解像度: 2.805 Å PDB-8wpt: Truncated mutant (1-171) of ferritin from Ureaplasma diversum 手法: X-RAY DIFFRACTION / 解像度: 2.36 Å PDB-8wpv: Truncated mutant (1-171) of ferritin from Ureaplasma diversum soaked in Fe2+ solution for 30min 手法: X-RAY DIFFRACTION / 解像度: 2.059 Å
化合物	ChemComp-FE: Unknown entry ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-CL: Unknown entry / クロリド ChemComp-HOH: WATER
由来	ureaplasma diversum (バクテリア)
キーワード	METAL BINDING PROTEIN / Ferritin / Iron / Fe-O cluster / 4-fold channel cavity / Ureaplasma diversum