EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	The step-by-step assembly mechanism of secreted flavivirus NS1 tetramer and hexamer captured at atomic resolution.
ジャーナル・号・ページ	Sci Adv, Vol. 10, Issue 18, Page eadm8275, Year 2024
掲載日	2024年5月3日
著者	Qi Pan / Haizhan Jiao / Wanqin Zhang / Qiang Chen / Geshu Zhang / Jianhai Yu / Wei Zhao / Hongli Hu /
PubMed 要旨	Flaviviruses encode a conserved, membrane-associated nonstructural protein 1 (NS1) with replication and immune evasion functions. The current knowledge of secreted NS1 (sNS1) oligomers is based on ...Flaviviruses encode a conserved, membrane-associated nonstructural protein 1 (NS1) with replication and immune evasion functions. The current knowledge of secreted NS1 (sNS1) oligomers is based on several low-resolution structures, thus hindering the development of drugs and vaccines against flaviviruses. Here, we revealed that recombinant sNS1 from flaviviruses exists in a dynamic equilibrium of dimer-tetramer-hexamer states. Two DENV4 hexameric NS1 structures and several tetrameric NS1 structures from multiple flaviviruses were solved at atomic resolution by cryo-EM. The stacking of the tetrameric NS1 and hexameric NS1 is facilitated by the hydrophobic β-roll and connector domains. Additionally, a triacylglycerol molecule located within the central cavity may play a role in stabilizing the hexamer. Based on differentiated interactions between the dimeric NS1, two distinct hexamer models (head-to-head and side-to-side hexamer) and the step-by-step assembly mechanisms of NS1 dimer into hexamer were proposed. We believe that our study sheds light on the understanding of the NS1 oligomerization and contributes to NS1-based therapies.
リンク	Sci Adv / PubMed:38691607 / PubMed Central
手法	EM (単粒子)
解像度	2.9 - 3.2 Å
構造データ	37419 8wbb EMDB-37419, PDB-8wbb: CryoEM structure of non-structural protein 1 dimer from dengue virus type 4 手法: EM (単粒子) / 解像度: 2.9 Å 37420 8wbc EMDB-37420, PDB-8wbc: CryoEM structure of non-structural protein 1 tetramer from dengue virus type 4 手法: EM (単粒子) / 解像度: 3.03 Å 37421 8wbd EMDB-37421, PDB-8wbd: CryoEM structure of non-structural protein 1 hexamer 1 from dengue virus type 4 手法: EM (単粒子) / 解像度: 2.9 Å 37422 8wbe EMDB-37422, PDB-8wbe: CryoEM structure of non-structural protein 1 hexamer 2 from dengue virus type 4 手法: EM (単粒子) / 解像度: 3.0 Å 37423 8wbf EMDB-37423, PDB-8wbf: CryoEM structure of non-structural protein 1 tetramer from ZIKA virus 手法: EM (単粒子) / 解像度: 2.99 Å 37424 8wbg EMDB-37424, PDB-8wbg: CryoEM structure of non-structural protein 1 tetramer from ZIKA virus 手法: EM (単粒子) / 解像度: 3.2 Å 37425 8wbh EMDB-37425, PDB-8wbh: CryoEM structure of non-structural protein 1 tetramer from Japanese encephalitis virus 手法: EM (単粒子) / 解像度: 3.2 Å
化合物	ChemComp-TGL: TRISTEAROYLGLYCEROL / トリステアリン / ステアリン
由来	dengue virus type 4 (デング熱ウイルス) dengue virus 4 philippines/h241/1956 (デング熱ウイルス) japanese encephalitis virus (日本脳炎ウイルス)
キーワード	VIRAL PROTEIN (ウイルスタンパク質) / flavivirus / non-structural protein 1 / dimer