EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Molecular insights into capsular polysaccharide secretion.
ジャーナル・号・ページ	Nature, Vol. 628, Issue 8009, Page 901-909, Year 2024
掲載日	2024年4月3日
著者	Jeremi Kuklewicz / Jochen Zimmer /
PubMed 要旨	Capsular polysaccharides (CPSs) fortify the cell boundaries of many commensal and pathogenic bacteria. Through the ABC-transporter-dependent biosynthesis pathway, CPSs are synthesized intracellularly ...Capsular polysaccharides (CPSs) fortify the cell boundaries of many commensal and pathogenic bacteria. Through the ABC-transporter-dependent biosynthesis pathway, CPSs are synthesized intracellularly on a lipid anchor and secreted across the cell envelope by the KpsMT ABC transporter associated with the KpsE and KpsD subunits. Here we use structural and functional studies to uncover crucial steps of CPS secretion in Gram-negative bacteria. We show that KpsMT has broad substrate specificity and is sufficient for the translocation of CPSs across the inner bacterial membrane, and we determine the cell surface organization and localization of CPSs using super-resolution fluorescence microscopy. Cryo-electron microscopy analyses of the KpsMT-KpsE complex in six different states reveal a KpsE-encaged ABC transporter, rigid-body conformational rearrangements of KpsMT during ATP hydrolysis and recognition of a glycolipid inside a membrane-exposed electropositive canyon. In vivo CPS secretion assays underscore the functional importance of canyon-lining basic residues. Combined, our analyses suggest a molecular model of CPS secretion by ABC transporters.
リンク	Nature / PubMed:38570679 / PubMed Central
手法	EM (単粒子)
解像度	3.1 - 4.4 Å
構造データ	41592 8tsh EMDB-41592, PDB-8tsh: S. thermodepolymerans KpsMT(E151Q)-KpsE in complex with ATP 手法: EM (単粒子) / 解像度: 3.1 Å 41593 8tsi EMDB-41593, PDB-8tsi: S. thermodepolymerans KpsMT-KpsE in complex with ADP:AlF4- 手法: EM (単粒子) / 解像度: 4.4 Å 41595 8tsl EMDB-41595, PDB-8tsl: S. thermodepolymerans KpsM-KpsE in Apo 2 state with rigid body fitted KpsT 手法: EM (単粒子) / 解像度: 3.4 Å 41601 8tsw EMDB-41601, PDB-8tsw: S. thermodepolymerans KpsMT-KpsE Apo 1 手法: EM (単粒子) / 解像度: 3.1 Å 41602 8tt3 EMDB-41602, PDB-8tt3: S. thermodepolymerans KpsM-KpsE in Glycolipid 2 state with rigid body fitted KpsT 手法: EM (単粒子) / 解像度: 3.4 Å 41626 8tun EMDB-41626, PDB-8tun: S. thermodepolymerans KpsM-KpsE in Glycolipid 1 state with rigid body fitted KpsT 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-41677: S. thermodepolymerans KpsMT-KpsE Apo 1 - consensus map 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-41678: S. thermodepolymerans KpsMT-KpsE Apo 1 - KpsT-focused map 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-41681: S. thermodepolymerans KpsMT(E151Q)-KpsE in complex with ATP - consensus map 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-41682: S. thermodepolymerans KpsMT(E151Q)-KpsE in complex with ATP - crown focused map 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-41697: S. thermodepolymerans KpsMT-KpsE in Apo 2 state - consensus map 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-41698: S. thermodepolymerans KpsMT-KpsE in Apo 2 state - KpsT focused map 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-41718: S. thermodepolymerans KpsMT-KpsE with bound glycolipid - state 1 - consensus map 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-41720: S. thermodepolymerans KpsMT-KpsE with bound glycolipid - state 1 - KpsT focused map 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-41721: S. thermodepolymerans KpsMT-KpsE with bound glycolipid - state 1 - KpsM focused map 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-41771: S. thermodepolymerans KpsMT-KpsE with bound glycolipid - state 2 - consensus map 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-41772: S. thermodepolymerans KpsMT-KpsE with bound glycolipid - state 2 - KpsT focused map 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-41773: S. thermodepolymerans KpsMT-KpsE with bound glycolipid - state 2 - KpsM focused map 手法: EM (単粒子) / 解像度: 3.4 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM / アデノシン三リン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-ALF: TETRAFLUOROALUMINATE ION / テトラフルオロアルミナ-ト化合物画像なし ChemComp-KJ9: Unknown entry
由来	caldimonas thermodepolymerans (バクテリア)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / ABC transporter / Capsular polysaccharide