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-Structure paper
タイトル | Structure of the intact tail machine of Anabaena myophage A-1(L). |
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ジャーナル・号・ページ | Nat Commun, Vol. 15, Issue 1, Page 2654, Year 2024 |
掲載日 | 2024年3月26日 |
著者 | Rong-Cheng Yu / Feng Yang / Hong-Yan Zhang / Pu Hou / Kang Du / Jie Zhu / Ning Cui / Xudong Xu / Yuxing Chen / Qiong Li / Cong-Zhao Zhou / |
PubMed 要旨 | The Myoviridae cyanophage A-1(L) specifically infects the model cyanobacteria Anabaena sp. PCC 7120. Following our recent report on the capsid structure of A-1(L), here we present the high-resolution ...The Myoviridae cyanophage A-1(L) specifically infects the model cyanobacteria Anabaena sp. PCC 7120. Following our recent report on the capsid structure of A-1(L), here we present the high-resolution cryo-EM structure of its intact tail machine including the neck, tail and attached fibers. Besides the dodecameric portal, the neck contains a canonical hexamer connected to a unique pentadecamer that anchors five extended bead-chain-like neck fibers. The 1045-Å-long contractile tail is composed of a helical bundle of tape measure proteins surrounded by a layer of tube proteins and a layer of sheath proteins, ended with a five-component baseplate. The six long and six short tail fibers are folded back pairwise, each with one end anchoring to the baseplate and the distal end pointing to the capsid. Structural analysis combined with biochemical assays further enable us to identify the dual hydrolytic activities of the baseplate hub, in addition to two host receptor binding domains in the tail fibers. Moreover, the structure of the intact A-1(L) also helps us to reannotate its genome. These findings will facilitate the application of A-1(L) as a chassis cyanophage in synthetic biology. |
リンク | Nat Commun / PubMed:38531972 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.26 - 3.9 Å |
構造データ | EMDB-37150, PDB-8ke9: EMDB-37151, PDB-8kea: EMDB-37152, PDB-8kec: EMDB-37153, PDB-8kee: EMDB-37154, PDB-8kef: EMDB-37155, PDB-8keg: EMDB-41590, PDB-8ts6: |
由来 |
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キーワード | VIRAL PROTEIN / fiber / virus / baseplate / tail / neck / portal |