EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural variation of types IV-A1- and IV-A3-mediated CRISPR interference.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 9306, Year 2024
掲載日	2024年10月29日
著者	R Čepaitė / N Klein / A Mikšys / S Camara-Wilpert / V Ragožius / F Benz / A Skorupskaitė / H Becker / G Žvejytė / N Steube / G K A Hochberg / L Randau / R Pinilla-Redondo / L Malinauskaitė / P Pausch /
PubMed 要旨	CRISPR-Cas mediated DNA-interference typically relies on sequence-specific binding and nucleolytic degradation of foreign genetic material. Type IV-A CRISPR-Cas systems diverge from this general ...CRISPR-Cas mediated DNA-interference typically relies on sequence-specific binding and nucleolytic degradation of foreign genetic material. Type IV-A CRISPR-Cas systems diverge from this general mechanism, using a nuclease-independent interference pathway to suppress gene expression for gene regulation and plasmid competition. To understand how the type IV-A system associated effector complex achieves this interference, we determine cryo-EM structures of two evolutionarily distinct type IV-A complexes (types IV-A1 and IV-A3) bound to cognate DNA-targets in the presence and absence of the type IV-A signature DinG effector helicase. The structures reveal how the effector complexes recognize the protospacer adjacent motif and target-strand DNA to form an R-loop structure. Additionally, we reveal differences between types IV-A1 and IV-A3 in DNA interactions and structural motifs that allow for in trans recruitment of DinG. Our study provides a detailed view of type IV-A mediated DNA-interference and presents a structural foundation for engineering type IV-A-based genome editing tools.
リンク	Nat Commun / PubMed:39468082 / PubMed Central
手法	EM (単粒子)
解像度	2.9 - 4.8 Å
構造データ	19045 8rc2 EMDB-19045, PDB-8rc2: DNA bound type IV-A3 CRISPR effector complex from K. pneumoniae 手法: EM (単粒子) / 解像度: 3.1 Å 19046 8rc3 EMDB-19046, PDB-8rc3: DNA bound type IV-A1 CRISPR effector complex from P. oleovorans 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-19120: DNA bound type IV-A1 CRISPR effector complex from P. oleovorans, main body 手法: EM (単粒子) / 解像度: 2.96 Å EMDB-19124: DNA bound type IV-A1 CRISPR effector complex from P. oleovorans Cas6 focused map 手法: EM (単粒子) / 解像度: 3.93 Å 19125 8rfj EMDB-19125, PDB-8rfj: DNA bound type IV-A1 CRISPR effector complex with the DinG helicase from P. oleovorans 手法: EM (単粒子) / 解像度: 3.18 Å EMDB-19126: Main density of the DNA bound type IV-A1 CRISPR effector complex with the DinG helicase from P. oleovorans 手法: EM (単粒子) / 解像度: 3.18 Å EMDB-19127: DinG focused map of DNA bound type IV-A1 CRISPR effector complex with the DinG helicase from P. oleovorans 手法: EM (単粒子) / 解像度: 4.18 Å 19688 8s35 EMDB-19688, PDB-8s35: DNA-bound Type IV-A3 CRISPR effector in complex with DinG helicase from K. pneumoniae (state I) 手法: EM (単粒子) / 解像度: 2.9 Å 19689 8s36 EMDB-19689, PDB-8s36: DNA-bound Type IV-A3 CRISPR effector in complex with DinG helicase from K. pneumoniae (state II) 手法: EM (単粒子) / 解像度: 2.9 Å 19690 8s37 EMDB-19690, PDB-8s37: DNA-bound Type IV-A3 CRISPR effector in complex with DinG helicase from K. pneumoniae (state III) 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-51026: Focused map of Cas6 of the CRISPR type IV-A1 DinG bound complex 手法: EM (単粒子) / 解像度: 4.8 Å
化合物	ChemComp-ZN: Unknown entry
由来	klebsiella pneumoniae (肺炎桿菌) pseudomonas oleovorans (バクテリア)
キーワード	GENE REGULATION / CRISPR / crRNA / DNA binding / type IV CRISPR-Cas / CRISPRi / nuclease deficient / ANTIVIRAL PROTEIN