EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Alternating L4 loop architecture of the bacterial polysaccharide co-polymerase WzzE.
ジャーナル・号・ページ	Commun Biol, Vol. 6, Issue 1, Page 802, Year 2023
掲載日	2023年8月2日
著者	Benjamin Wiseman / Göran Widmalm / Martin Högbom /
PubMed 要旨	Lipopolysaccharides such as the enterobacterial common antigen are important components of the enterobacterial cell envelope that act as a protective barrier against the environment and are often ...Lipopolysaccharides such as the enterobacterial common antigen are important components of the enterobacterial cell envelope that act as a protective barrier against the environment and are often polymerized by the inner membrane bound Wzy-dependent pathway. By employing cryo-electron microscopy we show that WzzE, the co-polymerase component of this pathway that is responsible for the length modulation of the enterobacterial common antigen, is octameric with alternating up-down conformations of its L4 loops. The alternating up-down nature of these essential loops, located at the top of the periplasmic bell, are modulated by clashing helical faces between adjacent protomers that flank the L4 loops around the octameric periplasmic bell. This alternating arrangement and a highly negatively charged binding face create a dynamic environment in which the polysaccharide chain is extended, and suggest a ratchet-type mechanism for polysaccharide elongation.
リンク	Commun Biol / PubMed:37532793 / PubMed Central
手法	EM (単粒子)
解像度	2.5 - 3.5 Å
構造データ	16071 8bhw EMDB-16071, PDB-8bhw: Full-length bacterial polysaccharide co-polymerase WzzE from E. coli. C4 symmetry 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-16072: Full-length bacterial polysaccharide co-polymerase WzzE from E. coli. C1 symmetry 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-16073: Full-length bacterial polysaccharide co-polymerase WzzE from E. coli. C8 symmetry 手法: EM (単粒子) / 解像度: 3.1 Å 17387 8p3o EMDB-17387, PDB-8p3o: Full-length bacterial polysaccharide co-polymerase WzzE mutant R267A from E. coli. C4 symmetry 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-17388: Full-length bacterial polysaccharide co-polymerase WzzE mutant R267A from E. coli. C1 symmetry 手法: EM (単粒子) / 解像度: 3.1 Å 17389 8p3p EMDB-17389, PDB-8p3p: Full-length bacterial polysaccharide co-polymerase WzzE mutant R267E from E. coli. C4 symmetry 手法: EM (単粒子) / 解像度: 2.5 Å EMDB-17390: Full-length bacterial polysaccharide co-polymerase WzzE mutant R267E from E. coli. C1 symmetry 手法: EM (単粒子) / 解像度: 2.7 Å
由来	escherichia coli k-12 (大腸菌)
キーワード	MEMBRANE PROTEIN / Complex / Lipopolysaccharide / bacterial polysaccharide co-polymerase