EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for activity switching in polymerases determining the fate of let-7 pre-miRNAs.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 31, Issue 9, Page 1426-1438, Year 2024
掲載日	2024年7月25日
著者	Gangshun Yi / Mingda Ye / Loic Carrique / Afaf El-Sagheer / Tom Brown / Chris J Norbury / Peijun Zhang / Robert J C Gilbert /
PubMed 要旨	Tumor-suppressor let-7 pre-microRNAs (miRNAs) are regulated by terminal uridylyltransferases TUT7 and TUT4 that either promote let-7 maturation by adding a single uridine nucleotide to the pre-miRNA ...Tumor-suppressor let-7 pre-microRNAs (miRNAs) are regulated by terminal uridylyltransferases TUT7 and TUT4 that either promote let-7 maturation by adding a single uridine nucleotide to the pre-miRNA 3' end or mark them for degradation by the addition of multiple uridines. Oligo-uridylation is increased in cells by enhanced TUT7/4 expression and especially by the RNA-binding pluripotency factor LIN28A. Using cryogenic electron microscopy, we captured high-resolution structures of active forms of TUT7 alone, of TUT7 plus pre-miRNA and of both TUT7 and TUT4 bound with pre-miRNA and LIN28A. Our structures reveal that pre-miRNAs engage the enzymes in fundamentally different ways depending on the presence of LIN28A, which clamps them onto the TUTs to enable processive 3' oligo-uridylation. This study reveals the molecular basis for mono- versus oligo-uridylation by TUT7/4, as determined by the presence of LIN28A, and thus their mechanism of action in the regulation of cell fate and in cancer.
リンク	Nat Struct Mol Biol / PubMed:39054354 / PubMed Central
手法	EM (単粒子)
解像度	3.65 - 4.0 Å
構造データ	16825 8oef EMDB-16825, PDB-8oef: Structure of human terminal uridylyltransferase 7 (hTUT7/ZCCHC6) 手法: EM (単粒子) / 解像度: 4.0 Å 17084 8opp EMDB-17084, PDB-8opp: Structure of human terminal uridylyltransferase 7 (hTUT7/ZCCHC6) bound with pre-let7g miRNA and UTPalphaS 手法: EM (単粒子) / 解像度: 3.76 Å 17086 8ops EMDB-17086, PDB-8ops: Human terminal uridylyltransferase 7 (TUT7/ZCCHC6) bound with pre-let7g miRNA and Lin28A - complex 1 手法: EM (単粒子) / 解像度: 3.82 Å 17087 8opt EMDB-17087, PDB-8opt: Human terminal uridylyltransferase 7 (TUT7/ZCCHC6) bound with pre-let7g miRNA and Lin28A - complex 2 手法: EM (単粒子) / 解像度: 3.65 Å 17164 8ost EMDB-17164, PDB-8ost: Structure of human terminal uridylyltransferase 4 (TUT4, ZCCHC11) in complex with pre-let7g miRNA and Lin28A 手法: EM (単粒子) / 解像度: 3.69 Å
化合物	ChemComp-P5E: [[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-sulfanyl-phosphoryl] phosphono hydrogen phosphate ChemComp-ZN: Unknown entry
由来	homo sapiens (ヒト)
キーワード	RNA / Polymerase / uridylation / RNA maturation and turnover control / RNA BINDING PROTEIN / CELL CYCLE