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-Structure paper
タイトル | Mechanism of heterochromatin remodeling revealed by the DDM1 bound nucleosome structures. |
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ジャーナル・号・ページ | Structure, Vol. 32, Issue 8, Page 1222-11230.e4, Year 2024 |
掲載日 | 2024年8月8日 |
著者 | Hongwei Zhang / Zhanxi Gu / Yuan Zeng / Yu Zhang / |
PubMed 要旨 | The SWI/SNF2 chromatin remodeling factor decreased DNA methylation 1 (DDM1) is essential for the silencing of transposable elements (TEs) in both euchromatic and heterochromatic regions. Here, we ...The SWI/SNF2 chromatin remodeling factor decreased DNA methylation 1 (DDM1) is essential for the silencing of transposable elements (TEs) in both euchromatic and heterochromatic regions. Here, we determined the cryo-EM structures of DDM1-nucleosome and DDM1-nucleosome complexes at near-atomic resolution in the presence of the ATP analog ADP-BeFx. The structures show that nucleosomal DNA is unwrapped more on the surface of the histone octamer containing histone H2A than that containing histone H2A.W. DDM1 embraces one DNA gyre of the nucleosome and interacts with the N-terminal tails of histone H4. Although we did not observe DDM1-H2A.W interactions in our structures, the results of the pull-down experiments suggest a direct interaction between DDM1 and the core region of histone H2A.W. Our work provides mechanistic insights into the heterochromatin remodeling process driven by DDM1 in plants. |
リンク | Structure / PubMed:38870940 |
手法 | EM (単粒子) |
解像度 | 3.1 - 3.17 Å |
構造データ | EMDB-37098, PDB-8kcb: EMDB-37099, PDB-8kcc: |
化合物 | ChemComp-BEF: ChemComp-ADP: |
由来 |
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キーワード | STRUCTURAL PROTEIN / DDM1 / nucleosome / H2A / H2A.W |