EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	An anti-CRISPR that represses its own transcription while blocking Cas9-target DNA binding.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 1806, Year 2024
掲載日	2024年2月28日
著者	Xieshuting Deng / Wei Sun / Xueyan Li / Jiuyu Wang / Zhi Cheng / Gang Sheng / Yanli Wang /
PubMed 要旨	AcrIIA15 is an anti-CRISPR (Acr) protein that inhibits Staphylococcus aureus Cas9 (SaCas9). Although previous studies suggested it has dual functions, the structural and biochemical basis for its two ...AcrIIA15 is an anti-CRISPR (Acr) protein that inhibits Staphylococcus aureus Cas9 (SaCas9). Although previous studies suggested it has dual functions, the structural and biochemical basis for its two activities remains unclear. Here, we determined the cryo-EM structure of AcrIIA15 in complex with SaCas9-sgRNA to reveal the inhibitory mechanism of the Acr's C-terminal domain (CTD) in mimicking dsDNA to block protospacer adjacent motif (PAM) recognition. For the N-terminal domain (NTD), our crystal structures of the AcrIIA15-promoter DNA show that AcrIIA15 dimerizes through its NTD to recognize double-stranded (ds) DNA. Further, AcrIIA15 can simultaneously bind to both SaCas9-sgRNA and promoter DNA, creating a supercomplex of two Cas9s bound to two CTDs converging on a dimer of the NTD bound to a dsDNA. These findings shed light on AcrIIA15's inhibitory mechanisms and its autoregulation of transcription, enhancing our understanding of phage-host interactions and CRISPR defense.
リンク	Nat Commun / PubMed:38418450 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.3 - 3.82 Å
構造データ	36217 8jft EMDB-36217, PDB-8jft: Cryo-EM structure of SaCas9-AcrIIA15 CTD-sgRNA complex 手法: EM (単粒子) / 解像度: 3.31 Å 36225 8jg9 EMDB-36225, PDB-8jg9: Cryo-EM structure of the SaCas9-sgRNA-AcrIIA15-promoter DNA dimer 手法: EM (単粒子) / 解像度: 3.82 Å PDB-8jfo: Crystal structure of anti-CRISPR protein AcrIIA15 手法: X-RAY DIFFRACTION / 解像度: 2.3 Å PDB-8jfr: N-terminal domain of AcrIIA15 in complex with palindromic DNA substrate 手法: X-RAY DIFFRACTION / 解像度: 3.1 Å PDB-8jfu: AcrIIA15 in complex with palindromic DNA substrate 手法: X-RAY DIFFRACTION / 解像度: 3.15 Å
化合物	ChemComp-HOH: WATER
由来	staphylococcus aureus (黄色ブドウ球菌) staphylococcus delphini (バクテリア)
キーワード	VIRAL PROTEIN / IIA type anti-crispr protein / II-A type anti-CRISPR protein / IIA type anti-crispr protein;