EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	An oligopeptide permease, OppABCD, requires an iron-sulfur cluster domain for functionality.
ジャーナル・号・ページ	Nat Struct Mol Biol, Year 2024
掲載日	2024年3月28日
著者	Xiaolin Yang / Tianyu Hu / Jingxi Liang / Zhiqi Xiong / Zhenli Lin / Yao Zhao / Xiaoting Zhou / Yan Gao / Shan Sun / Xiuna Yang / Luke W Guddat / Haitao Yang / Zihe Rao / Bing Zhang /
PubMed 要旨	Oligopeptide permease, OppABCD, belongs to the type I ABC transporter family. Its role is to import oligopeptides into bacteria for nutrient uptake and to modulate the host immune response. OppABCD ...Oligopeptide permease, OppABCD, belongs to the type I ABC transporter family. Its role is to import oligopeptides into bacteria for nutrient uptake and to modulate the host immune response. OppABCD consists of a cluster C substrate-binding protein (SBP), OppA, membrane-spanning OppB and OppC subunits, and an ATPase, OppD, that contains two nucleotide-binding domains (NBDs). Here, using cryo-electron microscopy, we determined the high-resolution structures of Mycobacterium tuberculosis OppABCD in the resting state, oligopeptide-bound pre-translocation state, AMPPNP-bound pre-catalytic intermediate state and ATP-bound catalytic intermediate state. The structures show an assembly of a cluster C SBP with its ABC translocator and a functionally required [4Fe-4S] cluster-binding domain in OppD. Moreover, the ATP-bound OppABCD structure has an outward-occluded conformation, although no substrate was observed in the transmembrane cavity. Here, we reveal an oligopeptide recognition and translocation mechanism of OppABCD, which provides a perspective on how this and other type I ABC importers facilitate bulk substrate transfer across the lipid bilayer.
リンク	Nat Struct Mol Biol / PubMed:38548954
手法	EM (単粒子) / X線回折
解像度	1.98 - 3.28 Å
構造データ	35990 8j5q EMDB-35990, PDB-8j5q: Cryo-EM structure of Mycobacterium tuberculosis OppABCD in the pre-translocation state 手法: EM (単粒子) / 解像度: 3.25 Å 35991 8j5r EMDB-35991, PDB-8j5r: Cryo-EM structure of Mycobacterium tuberculosis OppABCD in the resting state 手法: EM (単粒子) / 解像度: 3.28 Å 35992 8j5s EMDB-35992, PDB-8j5s: Cryo-EM structure of Mycobacterium tuberculosis OppABCD in the pre-catalytic intermediate state 手法: EM (単粒子) / 解像度: 3.0 Å 35993 8j5t EMDB-35993, PDB-8j5t: Cryo-EM structure of Mycobacterium tuberculosis OppABCD in the catalytic intermediate state 手法: EM (単粒子) / 解像度: 2.98 Å PDB-8j5u: Crystal structure of Mycobacterium tuberculosis OppA complexed with an endogenous oligopeptide 手法: X-RAY DIFFRACTION / 解像度: 1.98 Å
化合物	ChemComp-SF4: IRON/SULFUR CLUSTER / 鉄・硫黄クラスター ChemComp-ANP: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP / AMP-PNP, エネルギー貯蔵分子類似体YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM / アデノシン三リン酸 ChemComp-HOH: WATER / 水
由来	mycobacterium tuberculosis (strain atcc 25618 / h37rv) (結核菌) mycolicibacterium smegmatis mc2 155 (バクテリア)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / OppABCD / Type I ABC importer / Oligopeptide permease / Mycobacterium tuberculosis (結核菌) / AMPPNP-Mg / Pre-catalytic intermediate state / ATP-Mg / Occluded state / PEPTIDE BINDING PROTEIN / OppA / Oligopeptide binding protein / Cluster C SBP