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-Structure paper
タイトル | Structural and biochemical insights into the mechanism of the Gabija bacterial immunity system. |
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ジャーナル・号・ページ | Nat Commun, Vol. 15, Issue 1, Page 836, Year 2024 |
掲載日 | 2024年1月29日 |
![]() | Yanwu Huo / Lingfei Kong / Ye Zhang / Min Xiao / Kang Du / Sunyuntao Xu / Xiaoxue Yan / Jun Ma / Taotao Wei / ![]() |
PubMed 要旨 | The Gabija system is a newly discovered bacterial immune system that consists of GajA and GajB. Here we report the cryo-EM structure of the Gabija complex from Bacillus cereus VD045 at 3.6 Å, ...The Gabija system is a newly discovered bacterial immune system that consists of GajA and GajB. Here we report the cryo-EM structure of the Gabija complex from Bacillus cereus VD045 at 3.6 Å, which provides the direct evidence of interactions between GajA and GajB. The Gabija complex is an octameric ring structure with four GajA and four GajB. GajA is an OLD nucleases family protein, while GajB belongs to the SF1 helicases. The Gabija complex has sequence-specific DNA nuclease activity and prefers circular rather than linear DNA as substrate, its activity is more sensitive to concentrations change of nucleotides compared to GajA alone. Our data suggest a mechanism of Gabija immunity: the nuclease activity of Gabija complex is inhibited under physiological conditions, while it is activated by depletion of NTP and dNTP upon the replication and transcription of invading phages and cleave the circular DNA to prevent phage DNA replication. |
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手法 | EM (単粒子) |
解像度 | 3.6 Å |
構造データ | EMDB-35977, PDB-8j4t: |
由来 |
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![]() | DNA BINDING PROTEIN / Complex / DNA-binding / Immunity system |