EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structures of LHCII in photo-active and photo-protecting states reveal allosteric regulation of light harvesting and excess energy dissipation.
ジャーナル・号・ページ	Nat Plants, Vol. 9, Issue 9, Page 1547-1557, Year 2023
掲載日	2023年8月31日
著者	Meixia Ruan / Hao Li / Ying Zhang / Ruoqi Zhao / Jun Zhang / Yingjie Wang / Jiali Gao / Zhuan Wang / Yumei Wang / Dapeng Sun / Wei Ding / Yuxiang Weng /
PubMed 要旨	The major light-harvesting complex of photosystem II (LHCII) has a dual regulatory function in a process called non-photochemical quenching to avoid the formation of reactive oxygen. LHCII undergoes ...The major light-harvesting complex of photosystem II (LHCII) has a dual regulatory function in a process called non-photochemical quenching to avoid the formation of reactive oxygen. LHCII undergoes reversible conformation transitions to switch between a light-harvesting state for excited-state energy transfer and an energy-quenching state for dissipating excess energy under full sunshine. Here we report cryo-electron microscopy structures of LHCII in membrane nanodiscs, which mimic in vivo LHCII, and in detergent solution at pH 7.8 and 5.4, respectively. We found that, under low pH conditions, the salt bridges at the lumenal side of LHCII are broken, accompanied by the formation of two local α-helices on the lumen side. The formation of α-helices in turn triggers allosterically global protein conformational change, resulting in a smaller crossing angle between transmembrane helices. The fluorescence decay rates corresponding to different conformational states follow the Dexter energy transfer mechanism with a characteristic transition distance of 5.6 Å between Lut1 and Chl612. The experimental observations are consistent with the computed electronic coupling strengths using multistate density function theory.
リンク	Nat Plants / PubMed:37653340
手法	EM (単粒子)
解像度	2.52 - 2.8 Å
構造データ	35782 8iwx EMDB-35782, PDB-8iwx: Cryo-EM structure of unprotonated LHCII in detergent solution at high pH value 手法: EM (単粒子) / 解像度: 2.59 Å 35783 8iwy EMDB-35783, PDB-8iwy: Cryo-EM structure of protonated LHCII in detergent solution at low pH value 手法: EM (単粒子) / 解像度: 2.68 Å 35784 8iwz EMDB-35784, PDB-8iwz: Cryo-EM structure of unprotonated LHCII in detergent solution at low pH value 手法: EM (単粒子) / 解像度: 2.52 Å 35785 8ix0 EMDB-35785, PDB-8ix0: Cryo-EM structure of unprotonated LHCII nanodisc at high pH value 手法: EM (単粒子) / 解像度: 2.64 Å 35786 8ix1 EMDB-35786, PDB-8ix1: Cryo-EM structure of protonated LHCII nanodisc at low pH value 手法: EM (単粒子) / 解像度: 2.63 Å 35787 8ix2 EMDB-35787, PDB-8ix2: Cryo-EM structure of unprotonated LHCII nanodisc at low pH value 手法: EM (単粒子) / 解像度: 2.8 Å
化合物	ChemComp-CHL: CHLOROPHYLL B ChemComp-CLA: CHLOROPHYLL A ChemComp-LUT: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL ChemComp-XAT: (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL / 13-cis-ビオラキサンチン ChemComp-NEX: (1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL ChemComp-LHG: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / ジパルミトイルホスファチジルグリセロ-ル / リン脂質*YM
由来	spinacia oleracea (ホウレンソウ) Spinacia (ホウレンソウ)
キーワード	PLANT PROTEIN / LHCII / Light-harvesting complex / Photosystem II / Photosynthesis II / PHOTOSYNTHESIS