EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of white-tailed deer, , ACE2 recognizing all the SARS-CoV-2 variants of concern with high affinity.
ジャーナル・号・ページ	J Virol, Vol. 97, Issue 9, Page e0050523, Year 2023
掲載日	2023年9月28日
著者	Pu Han / Yumin Meng / Di Zhang / Zepeng Xu / Zhiyuan Li / Xiaoqian Pan / Zhennan Zhao / Linjie Li / Lingfeng Tang / Jianxun Qi / Kefang Liu / George F Gao /
PubMed 要旨	SARS-CoV-2 has been expanding its host range, among which the white-tailed deer (WTD), became the first wildlife species infected on a large scale and might serve as a host reservoir for variants of ...SARS-CoV-2 has been expanding its host range, among which the white-tailed deer (WTD), became the first wildlife species infected on a large scale and might serve as a host reservoir for variants of concern (VOCs) in case no longer circulating in humans. In this study, we comprehensively assessed the binding of the WTD angiotensin-converting enzyme 2 (ACE2) receptor to the spike (S) receptor-binding domains (RBDs) from the SARS-CoV-2 prototype (PT) strain and multiple variants. We found that WTD ACE2 could be broadly recognized by all of the tested RBDs. We further determined the complex structures of WTD ACE2 with PT, Omicron BA.1, and BA.4/5 S trimer. Detailed structural comparison revealed the important roles of RBD residues on 486, 498, and 501 sites for WTD ACE2 binding. This study deepens our understanding of the interspecies transmission mechanisms of SARS-CoV-2 and further addresses the importance of constant monitoring on SARS-CoV-2 infections in wild animals. IMPORTANCE Even if we manage to eliminate the virus among humans, it will still circulate among wildlife and continuously be transmitted back to humans. A recent study indicated that WTD may serve as reservoir for nearly extinct SARS-CoV-2 strains. Therefore, it is critical to evaluate the binding abilities of SARS-CoV-2 variants to the WTD ACE2 receptor and elucidate the molecular mechanisms of binding of the RBDs to assess the risk of spillback events.
リンク	J Virol / PubMed:37676003 / PubMed Central
手法	EM (単粒子)
解像度	2.55 - 3.51 Å
構造データ	34727 8hfx EMDB-34727, PDB-8hfx: Cryo-EM structure of SARS-CoV-2 Omicron BA.1 spike protein in complex with white-tailed deer ACE2 手法: EM (単粒子) / 解像度: 2.98 Å 34728 8hfy EMDB-34728, PDB-8hfy: SARS-CoV-2 Omicron BA.1 spike protein receptor-binding domain in complex with white-tailed deer ACE2 手法: EM (単粒子) / 解像度: 3.21 Å 34729 8hfz EMDB-34729, PDB-8hfz: Cryo-EM structure of SARS-CoV-2 prototype spike protein in complex with white-tailed deer ACE2 手法: EM (単粒子) / 解像度: 2.71 Å 34730 8hg0 EMDB-34730, PDB-8hg0: Cryo-EM structure of SARS-CoV-2 prototype spike protein receptor-binding domain in complex with white-tailed deer ACE2 手法: EM (単粒子) / 解像度: 3.51 Å 35426 8ify EMDB-35426, PDB-8ify: Cryo-EM structure of SARS-CoV-2 Omicron BA.4/5 spike protein in complex with white-tailed deer ACE2 手法: EM (単粒子) / 解像度: 2.55 Å 35427 8ifz EMDB-35427, PDB-8ifz: Cryo-EM structure of SARS-CoV-2 Omicron BA.4/5 spike protein receptor-binding domain in complex with white-tailed deer ACE2 手法: EM (単粒子) / 解像度: 2.85 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン ChemComp-ZN: Unknown entry
由来	severe acute respiratory syndrome coronavirus 2 (ウイルス) human immunodeficiency virus 1 (ヒト免疫不全ウイルス) odocoileus virginianus texanus (オジロジカ) odocoileus virginianus (オジロジカ)
キーワード	VIRAL PROTEIN / Complex / VIRAL PROTEIN/HYDROLASE / VIRAL PROTEIN-HYDROLASE complex