EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for DNA proofreading.
ジャーナル・号・ページ	Nat Commun, Vol. 14, Issue 1, Page 8501, Year 2023
掲載日	2023年12月27日
著者	Gina Buchel / Ashok R Nayak / Karl Herbine / Azadeh Sarfallah / Viktoriia O Sokolova / Angelica Zamudio-Ochoa / Dmitry Temiakov /
PubMed 要旨	DNA polymerase (DNAP) can correct errors in DNA during replication by proofreading, a process critical for cell viability. However, the mechanism by which an erroneously incorporated base ...DNA polymerase (DNAP) can correct errors in DNA during replication by proofreading, a process critical for cell viability. However, the mechanism by which an erroneously incorporated base translocates from the polymerase to the exonuclease site and the corrected DNA terminus returns has remained elusive. Here, we present an ensemble of nine high-resolution structures representing human mitochondrial DNA polymerase Gamma, Polγ, captured during consecutive proofreading steps. The structures reveal key events, including mismatched base recognition, its dissociation from the polymerase site, forward translocation of DNAP, alterations in DNA trajectory, repositioning and refolding of elements for primer separation, DNAP backtracking, and displacement of the mismatched base into the exonuclease site. Altogether, our findings suggest a conserved 'bolt-action' mechanism of proofreading based on iterative cycles of DNAP translocation without dissociation from the DNA, facilitating primer transfer between catalytic sites. Functional assays and mutagenesis corroborate this mechanism, connecting pathogenic mutations to crucial structural elements in proofreading steps.
リンク	Nat Commun / PubMed:38151585 / PubMed Central
手法	EM (単粒子)
解像度	2.58 - 3.08 Å
構造データ	29745 8g5i EMDB-29745, PDB-8g5i: Cryo-EM structure of the Mismatch Sensing Complex (I) of Human Mitochondrial DNA Polymerase Gamma 手法: EM (単粒子) / 解像度: 2.75 Å 29746 8g5j EMDB-29746, PDB-8g5j: Cryo-EM structure of the Mismatch Uncoupling Complex (II) of Human Mitochondrial DNA Polymerase Gamma 手法: EM (単粒子) / 解像度: 2.63 Å 29747 8g5k EMDB-29747, PDB-8g5k: Cryo-EM structure of the Wedge Alignment Complex (VIII) of Human Mitochondrial DNA Polymerase Gamma 手法: EM (単粒子) / 解像度: 2.9 Å 29748 8g5l EMDB-29748, PDB-8g5l: Cryo-EM structure of the Primer Separation Complex (IX) of Human Mitochondrial DNA Polymerase Gamma 手法: EM (単粒子) / 解像度: 3.0 Å 29749 8g5m EMDB-29749, PDB-8g5m: Cryo-EM structure of the Mismatch Locking Complex (III) of Human Mitochondrial DNA Polymerase Gamma 手法: EM (単粒子) / 解像度: 2.58 Å 29750 8g5n EMDB-29750, PDB-8g5n: Cryo-EM structure of the Guide loop Engagement Complex (VI) of Human Mitochondrial DNA Polymerase Gamma 手法: EM (単粒子) / 解像度: 2.73 Å 29751 8g5o EMDB-29751, PDB-8g5o: Cryo-EM structure of the Guide loop Engagement Complex (IV) of Human Mitochondrial DNA Polymerase Gamma 手法: EM (単粒子) / 解像度: 2.61 Å 29752 8g5p EMDB-29752, PDB-8g5p: Cryo-EM structure of the Guide loop Engagement Complex (V) of Human Mitochondrial DNA Polymerase Gamma 手法: EM (単粒子) / 解像度: 2.78 Å 41091 8t7e EMDB-41091, PDB-8t7e: Cryo-EM structure of the Backtracking Initiation Complex (VII) of Human Mitochondrial DNA Polymerase Gamma 手法: EM (単粒子) / 解像度: 3.08 Å
由来	homo sapiens (ヒト) synthetic construct (人工物) synthetic rna (人工物)
キーワード	REPLICATION/DNA / Mitochondrial DNA Polymerase / DNA Proofreading / Mismatch Sensing / REPLICATION / REPLICATION-DNA complex / PolG / Wedge alignment / Primer Separation / REPLICATION/DNA/RNA / Mismatch Locking / REPLICATION-DNA-RNA complex / Guide loop Engagement / Transferase/DNA / Backtracking / Mismatch repair / Transferase-DNA complex