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-Structure paper
タイトル | Production and characterization of an AAV1-VP3-only capsid: An analytical benchmark standard. |
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ジャーナル・号・ページ | Mol Ther Methods Clin Dev, Vol. 29, Page 460-472, Year 2023 |
掲載日 | 2023年6月8日 |
著者 | Mario Mietzsch / Weijing Liu / Ke Ma / Antonette Bennett / Austin R Nelson / Keely Gliwa / Paul Chipman / Xiaofeng Fu / Shane Bechler / Robert McKenna / Rosa Viner / |
PubMed 要旨 | Adeno-associated viruses (AAVs) are non-enveloped ssDNA icosahedral T = 1 viruses used as vectors for clinical gene delivery. Currently, there are over 200 AAV-related clinical trials and six ...Adeno-associated viruses (AAVs) are non-enveloped ssDNA icosahedral T = 1 viruses used as vectors for clinical gene delivery. Currently, there are over 200 AAV-related clinical trials and six approved biologics on the market. As such new analytical methods are continually being developed to characterize and monitor the quality and purity of manufactured AAV vectors, these include ion-exchange chromatography and Direct Mass Technology. However, these methods require homogeneous analytical standards with a high molecular weight standard comparable to the mass of an AAV capsid. Described here is the design, production, purification, characterization, and the cryo-electron microscopy structure of an AAV1-VP3-only capsid that fulfills this need as a calibrant to determine capsid mass, charge, homogeneity, and transgene packaging characteristics. |
リンク | Mol Ther Methods Clin Dev / PubMed:37273903 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.27 Å |
構造データ | EMDB-29377, PDB-8fq4: |
由来 |
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キーワード | VIRUS LIKE PARTICLE / AAV / gene therapy / capsid / VP3 |